<p style="text-align: justify;">Transgenic TC1507 maize was produced by biolistic (microprojectile bombardment) transformation of the hybrid maize line Hi-II (Hi-II is a cross between A188 and B73 inbred lines of maize) with plasmid DNA containing sequences corresponding to a modified (synthetic, less than full-length) form of the <i>cry1Fa2</i> gene from <i>Bacillus thuringiensis</i> var. <i>aizawai</i> strain PS811 and the phosphinothricin N-acetyltransferase (PAT) encoding gene from <i>Streptomyces viridochromogenes</i>.</p>

<p style="text-align: justify;">In order to optimize expression of the Cry1F protein, the nucleotide sequence of the <i>cry1Fa2</i> gene was modified via <i>in vitro</i> mutagenesis to contain plant-preferred codons. Transcription of the <i>cr1Fa2</i> gene was directed by the promoter and a 5&#39; untranslated region from the maize ubiquitin (<i>ubi</i>) gene including the first exon and intron. The 3&#39; termination/polyadenylation sequences were derived from the <i>Agrobacterium tumefaciens</i> open reading frame 25 (ORF25 PolyA). The <i>ubi</i> exon and intron included in this construct (PHI8999) have no effect on the structure of the Cry1F product, only on the expression of the gene.</p>

<p style="text-align: justify;">Transcriptional regulation of the <i>pat</i> gene was via promter and terminator sequences derived from the 35S transcript of cauliflower mosaic virus (CaMV).</p>
