<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">Data from Southern blot analysis of genomic DNA from transgenic tomatoes supported the conclusion that a single copy of the T-DNA was integrated into a single site within the host genome.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The transformation events B, Da and F lead to the decrease (downregulation) of endogenous PG enzyme in fruit, at the onset of ripening. The only new protein expressed was NPTII, used as a selectable marker, which was detected in raw tomatoes from two of the hybrid lines derived from transformation event F, 1401F and H282F, at levels of 2.63 &plusmn; 0.89 &micro;g/mg and 3.46 &plusmn; 0.72 &micro;g/mg fresh tissue, respectively, but was not detected in tomato sauce following processing of either of these hybrids.</p>
