<p style="text-align: justify;">DAS-59122-7 X TC1507 X NK603 (OECD identifier: DAS-59122-7 X DAS-&Oslash;15&Oslash;7-1 X MON-00603-6) maize is a hybrid resulting from crossing the coleopteran-resistance maize line DAS-59122-7 with the lepidoteran-resistant maize line TC1507 and the herbicide-tolerant maize line NK603. This stacked maize hybrid is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment. Examples of jurisdictions that require either notification or information about stacked hybrids prior to their release into the environment, and for use in human food and livestock feed are Japan and Korea.</p>

<p style="text-align: justify;">The stacked hybrid DAS-59122-7 X TC1507 X NK603 expresses five novel proteins: the insect control proteins (delta-endotoxins) Cry34Ab1 and Cry35Ab1, which are insecticidal to coleopterans such as the Corn Rootworm, and Cry1F which confers resistance to the European Corn Borer and other lepidopterans, together with the PAT protein which confers tolerance to the herbicide glufosinate ammonium, and the CP4 EPSPS protein which confers resistance to the herbicide glyphosate. The insecticidal proteins Cry34Ab1 and Cry35Ab1 are produced by the <i>cry</i>34Ab1 and <i>cry</i>35Ab1 genes from DAS-59122-7. The Cry1F insecticidal protein is produced by the <i>cry</i>1F gene from TC1507 and PAT is produced by the <i>pat</i> gene, contained in both DAS-59122-7 and TC1507. The CP4 EPSPS protein is produced by the <i>cp4 epsps</i> gene from NK603. The novel traits of each parental line have been combined, through traditional plant breeding, to produce this new hybrid. For a full description of each parental line please refer to the individual product descriptions in the crop database for DAS-59122-7, TC1507 and NK603.</p>

<p style="text-align: justify;">The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid. The Cry34Ab1, Cry35Ab1 and Cry1F proteins are not enzymes and therefore do not affect plant metabolism. Both the PAT and CP4 EPSPS have high substrate affinities: PAT for L-Phosphinothricin, the active ingredient in glufosinate ammonium, and CP4 EPSPS for phosphoenolpyruvate (PEP) and shikimate-3-phosphate, which are part of the shikimate metabolic pathway. Since both PAT and CP4 EPSPS differ in substrates and in mode of action, these are not expected to interact within, nor affect the metabolism of the stacked hybrid.</p>
