<p style="text-align: justify;"><b>Genetic Stability of the Introduced Trait</b></p>

<p style="text-align: justify;">Maize line MON80100 has been field tested since 1992 in the major maize growing regions of the United States. Southern Blot analysis of genomic DNA from 5 generations of backcrosses of the MON80100 line to the B73 parental line and 4 generations of crosses to an unrelated inbred line confirmed stable inheritance of the <i>cry1Ab</i> gene. Southern blot and segregation analyses indicated that the introduced genes were stably integrated into the maize genome and followed Mendelian rules of inheritance.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The synthetic <i>cry1Ab</i> gene was linked to a strong constitutive promoter and modified for maximum expression in maize. The endotoxin expressed in the modified maize line MON80100 was found to be identical to that occurring naturally, and equivalent to the active ingredient in microbial formulations of B.t.k. spray. The expression levels of Cry1Ab protein, CP4 EPSPS and glyphosate oxidoreductase were determined in the leaves, kernels and pollen of transgenic maize. In leaves and kernels, the amounts of Cry1Ab and CP4 EPSPS were to be 1.3 or 0.57 &Acirc;&micro;g/g leaf fresh weight and 1.85 or 4.11 &Acirc;&micro;g/g kernel fresh weight, respectively. There were no detectable amounts of Cry1Ab protein in the pollen from transgenic MON80100 maize. Also, glyphosate oxidoreductase and NPTII were not detected in either leaves or kernels from MON80100 maize.</p>
