<p style="text-align: justify;">The transgenic tomato lines B, Da and F were produced via <i>Agrobacterium</i>-mediated transformation of the inbred tomato line TGT7 (T7) (<i>Lycopersicon esculentum</i> var. esculentum). This transformation system was based on the disarmed binary vector Bin19, which was engineered to contain DNA sequences encoding a partial copy of the PG gene, as well as the NPTII gene sequences. During transformation, the T-DNA portion of the plasmid was transferred into the plant cells and stably integrated into the plant&#39;s genome.</p>

<p style="text-align: justify;">The PG encoding gene was isolated from the tomato cultivar &lsquo;Ailsa Craig&rsquo; and was inserted into the Ti binary vector in either the sense or antisense orientation, in each case under the control of the cauliflower mosaic virus (CaMV) 35S promoter and termination sequences from the nopaline synthase gene (3&rsquo; <i>nos</i>) from <i>A. tumefaciens</i>. The NPTII encoding gene was isolated from the Tn5 transposon of <i>E. coli</i> and its expression was regulated using <i>nos</i> promoter and octopine synthase terminator sequences from <i>A. tumefaciens</i>.</p>

<p style="text-align: justify;">Tomato line F was further described to include the following DNA sequence elements; the origin of replication and internal fragment of gene III from bacteriophage M13, two <i>lac</i>Z gene fragments from <i>E. coli</i> and an <i>ocd</i> gene (encoding ornithine cyclodeaminase) from <i>A. tumefaciens</i>.</p>

<p style="text-align: justify;">Tomato line F was used to develop the tomato hybrids 1401F, H282F, 11013F and 7913F.</p>
