<p style="text-align: justify;">Canola line HCN92 was produced by <i>Agrobacterium</i>-mediated transformation of microspores from <i>B. napus</i> cultivar &#39;Topas&#39;. The T-DNA region of the Ti plasmid was &quot;disarmed&quot; by removal of <i>vir</i>A genes, normally associated with the pathogenicity and disease-causing properties of <i>A. tumefaciens</i>, and replaced with the genes coding for glufosinate ammonium tolerance and aminoglycoside resistance. During transformation, the T-DNA portion of the plasmid was transferred into the plant cells and stably integrated into the plant&#39;s genome.</p>

<p style="text-align: justify;">The original transformant (Topas 19/2) was produced using plasmid pOCA/Ac and contained a synthetic <i>pat</i> gene coding for phosphinothricin acetyltransferase (PAT) and the aminoglycoside resistance marker <i>neo</i> coding for neomycin phosphotransferase II. Expression of the <i>pat</i> gene was regulated by the cauliflower mosaic virus (CaMV) 35S promoter and terminator sequences. The <i>neo</i> gene was driven by the nopaline synthase promoter and an octopine synthase terminator sequence.</p>

<p style="text-align: justify;">HCN92 was derived from traditional breeding crosses between non-genetically modified canola and a line resulting from transformation event (Topas 19/2). The transformed line was initially crossed with line ACSN3, followed by a second cross with cultivar &#39;AC Excel&#39;. The seed from the final cross was advanced and single seed from F3 plants were bulked to form HCN92.</p>
