<p style="text-align: justify;">TC-6275 maize was produced by <i>Agrobacterium</i>-mediated transformation of the inbred maize line Hi-II with the plasmid vector PHP12537. The T-DNA segment of PHP12537 contained sequences of a modified (<i>i.e.</i>, synthetic, maize-optimized and truncated) form of the <i>cry</i>1F gene (mo<i>cry</i>1F) from <i>Bacillus thuringiensis</i> var. <i>aizawai</i> strain PS811, and the phosphinothricin N-acetyltransferase (PAT) encoding <i>bar</i> gene from <i>Streptomyces hygroscopicus</i>. The nucleotide sequence of the <i>cry</i>1F gene was modified to contain codons to optimize expression of the Cry1F protein in maize. Transcription of the <i>cry</i>1F gene was directed by the promoter, intron and 5&#39; untranslated sequences from the maize ubiquitin gene. Terminator sequences were derived from the <i>Solanum tuberosum</i> (potato) proteinase inhibitor II (PINII). The expression of the <i>bar</i> gene was regulated by the 35S promoter and upstream enhancer sequences from the Cauliflower Mosaic Virus (CaMV) strain 1841, and the alcohol dehydrogenase intron (ADH1) from <i>Zea mays</i>. Terminator sequences were derived from the <i>Solanum tuberosum</i> proteinase inhibitor II.</p>
