<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">EH92-527-1 contains a single insert. DNA sequences towards the left border region of the T-DNA were deleted and the entire T-DNA insert, including the flanking region, was duplicated in reverse orientation. The insert includes the 5&#39; untranslated region of the nopaline synthase gene (Pnos), the <i>nptII</i> coding sequence, the 3&#39; untranslated part of the nopaline synthase gene, the potato <i>gbss</i> promoter fragment, and a truncated <i>gbss </i>coding region in antisense orientation without the terminator. The insert, together with the potato flanking region, was duplicated in a tail-to-tail arrangement with the two right border regions as junctions to the potato chromosomal DNA. Southern blot analyses indicated that no vector backbone sequences were inserted into potato EH92-527-1. No other unknown DNA sequences have been recombined into the inserted T-DNA.</p>

<p style="text-align: justify;"><b>Genetic Stability of the Introduced Trait</b></p>

<p style="text-align: justify;">The stability of the inserted DNA in potato EH92-527-1 was assessed over several cycles of vegetative propagation via cuttings and tubers. The inserted trait was found to be stable over multiple generations based on compositional analyses of tuber starch from EH92-527-1. Southern blot analyses demonstrated that genomic DNA isolated in 1998 and 2005 had similar hybridization patterns with four different restriction enzyme combinations.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The antisense orientation of the introduced gbss gene relative to the <i>gbss</i> promoter leads to a decrease in amylose content and an associated increase in amylopectin content. In conventional potato starch, the GBSS protein represents approximately 80% of the extractable protein; in EH92-527-1, the GBSS protein was not detectable.</p>

<p style="text-align: justify;">NPTII protein expression was analyzed in leaves, tubers, pulp and starch. NPTII levels were 0.00082% of the soluble protein in pulp (8.2 ng/mg total protein; 55 ng/g fresh weight) and 0.0006% in tubers (6.82 ng/mg protein; 31 ng/g fresh weight). NPTII was much lower in leaves and undetectable in starch.</p>
