<p style="text-align: justify;">Sugar beet line H7-1 was produced by <i>Agrobacterium</i>-mediated transformation of the sugar beet line 3S0057 with the binary plasmid vector PV-BVGT08. The T-DNA region of the plasmid vector contained sequences encoding CP4 EPSPS from <i>Agrobacterium</i> sp. strain CP4. Associated with the CP4 EPSPS encoding gene were modified promoter sequences from the 35S transcript of figwort mosaic virus (P-FMV), sequences encoding the chloroplast transit peptide (<i>ctp2</i>) from the <i>Arabidopsis thaliana</i> EPSPS gene, and transcription termination and polyadenylation signal sequences from the ribulose-1,5-bisphosphate carboxylase small subunit (<i>rbcS</i>) E9 encoding gene from pea (<i>Pisum sativum</i>). Right border and left border sequences of the T-DNA region of the plasmid vector were derived from <i>Agrobacterium tumefaciens</i>. The plasmid also contained a vegetative and bacterial origin of replication for maintenance and replication of the plasmid in both <i>A. tumefaciens</i> and <i>E. coli</i>. The <i>aad</i> gene, encoding the Tn7 AAD 3&rsquo; adenyltransferase that provides resistance to spectinomicyn and streptomycin, was also present in the plasmid, as well as <i>rop</i>, a sequence that helps to maintain and control the copy number of the plasmid in <i>E. coli</i>.</p>

<p style="text-align: justify;">The plasmid vector PV-BVGT08 did not contain marker gene sequences. The selection of successfully transformed cells was achieved by applying glyphosate and screening for tolerant cells.</p>
