<p style="text-align: justify;">The wheat line MON 71800 was produced by <i>Agrobacterium</i>-mediated transformation of plant cells from &lsquo;Bobwhite&rsquo; spring wheat. The plasmid vector PV-TXGT10 used for the transformation contained two <i>cp4 epsps</i> gene cassettes coding for glyphosate tolerance. Each gene cassette consisted of chloroplast transit peptide coding sequences from the <i>Arabidopsis thaliana epsps</i> gene (<i>Arab</i>TP) associated with the sequences of the <i>cp4 epsps</i> gene. Two different promoters were used to regulate the expression of each <i>cp4 epsps</i> gene: 1) the enhanced 35S promoter from the cauliflower mosaic virus and 2), the promoter, transcription start site, and first intron of the 5&rsquo; region of the rice <i>actin</i>1 gene. Terminator sequences in each gene casette consisted of the 3&rsquo; non-translated region of the nopaline synthase gene (nos 3&rsquo;). The PV-TXGT10 vector backbone contained the origin of replication sequences <i>ori-V</i> and <i>ori-322/rop</i>. The vector backbone also contained the <i>aad</i> gene, which codes for streptomycin adenyltransferase, to allow the selection of bacteria containing the PV-TXGT10 vector.</p>
