<p style="text-align: justify;">The soybean line GU262 was produced via biolistic transformation of a soybean line with a pUC19 based plasmid vector, pB2/35SacK, containing the pat gene and sequences encoding the beta-lactamase enzyme that confers resistance to beta-lactam antibiotics such as ampicillin. Expression of the <i>pat</i> gene was regulated by including promoter and terminator sequences from the 35S transcript of cauliflower mosaic virus (CaMV).</p>

<p style="text-align: justify;">The <i>bla</i> gene, encoding beta-lactamase, was employed as a selection method during the development process in order to identify bacterial colonies that had been transformed with recombinant plasmids. The promoter sequences for the <i>bla</i> gene were only active in bacteria and there was no expression of these sequences in the modified soybean lines.</p>
