<p style="text-align: justify;">The 23-198 and 23-18-17 lines were created by <i>Agrobacterium</i>-mediated transformation of line 212/86 in which the transfer-DNA (T-DNA) contained the gene encoding the enzyme 12:0 ACP thioesterase (bay TE) from the California bay tree (<i>Umbellularia californica</i>). Expression of the bay TE gene was under the regulation of a seed specific plant promoter. In addition, the T-DNA contained sequences encoding the enzyme neomycin phosphotransferase II (NPTII). The expression of NPTII activity, regulated by the CaMV 35S promoter, was used as a selectable trait to screen transformed plants for the presence of the bay TE gene. No other translatable DNA sequences were incorporated into the plant genome.</p>
