<p style="text-align: justify;">Event 176 was produced by biolistic transformation of the inbred line CG00526 with two plasmids. One plasmid contained two copies of a 3&rsquo; truncated <i>cry1Ab</i> gene, each regulated by different promoter sequences. The <i>cry1Ab</i> open reading frame, corresponding to the sequence encoding the N-terminal 648 amino acids of the native Cry1Ab protein, was modified for optimal expression in plant cells. Green tissue expression of one copy of the <i>cry1Ab</i> gene was regulated by the phosphoenolpyruvate carboxylase promoter while expression of the other <i>cry1Ab</i> gene was controlled by a pollen specific promoter isolated from maize. Both genes employed 3&rsquo;-polyadenylation sequences from the 35S transcript of cauliflower mosaic virus (CaMV). This plasmid also contained a copy of the beta-lactamase encoding <i>bla</i> gene under control of a bacterial promoter. The <i>bla</i> gene was not expressed in plant cells, but was employed as a selectable trait for screening bacterial colonies for the presence of the plasmid vector. The second plasmid contained a copy of the bar gene from the soil bacterium <i>Streptomyces hygroscopicus</i> which encodes the phosphinothricin N-acetyl transferase (PAT) enzyme. This enzyme is used as a selectable marker and confers resistance to glufosinate ammonium herbicide. Constitutive expression of the <i>bar</i> gene was under the control of the CaMV 35S promoter. Apart from the sequences encoding Cry1Ab and PAT, no other plant translatable DNA sequences were introduced into the plant genome.</p>
