<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">Southern blot analysis of the genomic DNA of MON 88017 demonstrated the integration of a single, intact copy of the T-DNA of PV-ZMIR39. Both novel genes, <i>cry</i>3Bb1 and <i>cp4 epsps</i>, along with their respective promoter, enhancer and terminator sequences, were completely integrated. None of the vector backbone sequences, including the spectinomycin resistance and streptomycin resistance genes, were integrated into the genome of MON88017.</p>

<p style="text-align: justify;"><b>Genetic Stability of the Trait</b></p>

<p style="text-align: justify;">The stability of both <i>cry</i>3Bb1 and <i>cp4 epsps</i> genes were assessed using Southern blot analysis and segregation analysis across 10 generations. The integration was shown to be stable across generations and the genes segregated according to a Mendelian inheritance pattern.</p>
