The 45A37 and 46A40 lines of canola (Brassica napus) were developed through the combination of chemical mutagenesis, to achieve the high oleic acid trait, and traditional breeding with the registered canola varieties Stellar and Apollo, to achieve the low linolenic acid trait. The processed oil derived from these novel varieties, designated P6 canola oil, has levels of oleic acid similar to that of peanut and olive oils. <br>The high oleic acid trait in lines 45A37 and 46A40 was selected following chemical mutagenesis by exposing seeds of canola varieties Regent, Topas and Andor to a solution of ethylnitrosourea (8 mM) in dimethylsulfoxide. Ethylnitrosurea is a commonly used chemical mutagen that affects DNA by chemically altering base pairs. It is believed that the induced mutation in lines 45A37 and 46A40 is analogous to that in fad2 mutants of Arabidopsis thaliana. The fad2 gene encodes a desaturase enzyme that catalyzes the conversion of C18:1 to C18:2 and C18:3 fatty acids in plant cells. A mutation within the fad2 gene that blocks expression of an active desaturase enzyme results in the accumulation of C18:1 oleic acid. The low linolenic acid trait was introduced by breeding with the registered low linolenic acid canola varieties Stellar and Apollo. The only significant difference between lines 45A37 and 46A40 is that the former was selected for early maturity while 46A40 matures later in the growing season (medium maturity). <br>Based on the information provided, there are no novel proteins produced and only the refined seed oil will be used as a food. Refined edible canola oil does not contain any detectable protein and consists of purified glycosides. Other than the traits of high oleic acid content and low linolenic acid content in the seed oil, the disease, pest and other agronomic characteristics of the 45A37 and 46A40 canola lines were comparable to other commercially available canola varieties.