<p style="text-align: justify;">MIR162 maize was produced by <i>Agrobacterium tumefaciens</i>-mediated transformation of immature embryos using the plasmid vector pNOV1300. The transformation vector is a disarmed, binary vector that contains both left and right transfer-DNA (T-DNA) border sequences to facilitate transformation. The vector region between the left and right border sequences, which included the <i>vip3Aa19</i> and <i>pmi</i> gene expression cassettes, was inserted into the maize genome during transformation. One expression cassette consists of the <i>vip3Aa20</i> (see below for explanation of name change) coding region regulated by the <i>Zea mays</i> polyubiquitin promoter (ZmUbilInt) and cauliflower mosaic virus 35S 3&rsquo; polyadenylation sequences. A second expression cassette consists of the <i>pmi</i> coding region regulated by the <i>Zea mays</i> polyubiquitin promoter (ZmUbilInt) and the nopaline synthase (NOS) polyadenylation sequence.</p>
