<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">Southern blot analysis of the genomic DNA of DAS-59122-7 demonstrated the integration of a single intact copy of the T-DNA of the vector plasmid PHP17662. All three novel genes, <i>cry</i>34Ab1, <i>cry</i>35Ab1, and <i>pat</i>, along with their respective promoter, enhancer and terminator sequences, were completely integrated. None of the vector backbone sequences, including the spectinomycin resistance and tetracycline resistance genes, were integrated into the genome of DAS-59122-7.</p>

<p style="text-align: justify;"><b>Genetic Stability of the Trait</b></p>

<p style="text-align: justify;">Southern blot analysis demonstrated that the integration of the T-DNA insert was stable within and across generations. Segregation analysis also demonstrated a stable insertion consistent with a Mendelian inheritance pattern for a dominant trait.</p>
