<p>Maize line 4114 was produced by&nbsp;Agrobacterium tumefaciens-mediated transformation with plasmid PHP27118 containing the&nbsp;<em>cry1F</em>, <em>cry34Ab1</em>, <em>cry35Ab1</em>,&nbsp;and&nbsp;<em>pat</em>&nbsp;gene expression cassettes. These gene expression cassettes are exactly the same as used in the production of 1507 maize (<em>cry1F</em>, <em>pat</em>) and 59122 maize (<em>cry34Ab1</em>, <em>cry35Ab1</em>, <em>pat</em>). The first cassette contains a truncated version of the&nbsp;<em>cry1F</em>&nbsp;gene from&nbsp;<em>Bacillus thuringiensis</em>&nbsp;var.&nbsp;<em>aizawai</em>. Transcriptional control is provided by the maize polyubiquitin promoter, resulting in constitutive expression of the Cry1F protein in maize. This region also includes the 5&#39; untranslated region (UTR) and intron associated with the native polyubiquitin promoter. The terminator for the&nbsp;<em>cry1F</em>&nbsp;gene is the polyadenylation signal from open reading frame 25 (ORF25) of the&nbsp;<em>A. tumefaciens</em>&nbsp;Ti plasmid pTi15955.<br>The second cassette contains the&nbsp;cry34Ab1&nbsp;gene isolated from&nbsp;B. thuringiensis&nbsp;strain PS149B1. The expression of the&nbsp;cry34Ab1&nbsp;gene is controlled by a second copy of the maize polyubiquitin promoter with 5&#39; UTR and intron. The terminator for the&nbsp;cry34Ab1gene is the 3&rsquo; terminator sequence from the proteinase inhibitor II gene (pinIIterminator) of&nbsp;Solanum tuberosum.<br>The third gene cassette contains the&nbsp;cry35Ab1&nbsp;gene, also isolated from&nbsp;B. thuringiensisstrain PS149B1. The expression of the&nbsp;cry35Ab1&nbsp;gene is controlled by the&nbsp;Triticum aestivum&nbsp;(wheat) peroxidase promoter and leader sequence. The terminator for thecry35Ab1&nbsp;gene is a second copy of the&nbsp;pinII&nbsp;terminator.<br>The fourth and final gene cassette contains a version of the phosphinothricin N-acetyltransferase (pat) gene from&nbsp;Streptomyces viridochromogenes&nbsp;that has been optimized for expression in plants. Expression of the&nbsp;pat&nbsp;gene is controlled by the promoter and terminator regions from the cauliflower mosaic virus (CaMV) 35S transcript.</p><br>