<p style="text-align: justify;">Soybean line GTS 40-3-2 was produced by biolistic transformation of plant cells from soybean cultivar A5403 with DNA-coated gold particles. The plasmid PV-GMGT04 used for transformation contained the genes coding for glyphosate tolerance and for production of &szlig;-glucuronidase (<i>gus</i> gene), a selectable marker. Expression of the CP4 EPSPS gene was regulated by an enhanced 35S promoter (E35S) from cauliflower mosaic virus (CaMV), a chloroplast transit peptide (CTP4) coding sequence from <i>Petunia hybrida</i>, and a nopaline synthase (<i>nos</i> 3&#39;) transcriptional termination element from <i>Agrobacterium tumefaciens</i>. The transit peptide facilitated the translocation of newly translated EPSP synthase into chloroplasts, the site of aromatic amino acid biosynthesis and glyphosate site of action.</p>
