<p style="text-align: justify;">Events J101 and J163 were developed by <i>Agrobacterium</i>-mediated transformation of plant cells from the proprietary alfalfa clone &lsquo;R2336.&rsquo; Plant transformation was achieved with the plasmid vector PV-MSHT4. The T-DNA portion of this plasmid vector contained one <i>cp4 epsps</i> gene expression cassette coding for glyphosate tolerance. The <i>cp4 epsps</i> gene was linked to a chloroplast transit peptide (<i>cpt2</i>) sequence, derived from the <i>Arabidopsis thaliana epsps</i> gene. The expression of the <i>cp4 epsps</i> gene was regulated by an enhanced 35S promoter from the Figwort Mosaic Virus, and the petunia heat shock protein 70 5&rsquo; untranslated leader sequence. The transcriptional termination sequences were derived from the pea (<i>Pisum sativum</i>) ribulose-1,5-biphosphate carboxylase (Rubisco) small subunit E9 gene.</p>
