<p style="text-align: justify;">The transgenic tomato line 1345-4 was produced by <i>Agrobacterium</i>-mediated transformation of the parental line 91103-114 (<i>Lycopersicon esculentum</i> var. esculentum) in which the transfer-DNA (T-DNA) region of the bacterial tumour inducing (Ti) plasmid was modified to contain DNA sequences encoding a truncated ACC synthase gene construct from the tomato cultivar &lsquo;Golden Nugget&rsquo; and the NPTII encoding <i>neo</i> gene from <i>E. coli</i> strain K12. During transformation, the T-DNA portion of the plasmid was transferred into the plant cells and stably integrated into the plant&#39;s genome. Expression of the ACC synthase gene was under the regulatory control of the 35S promoter from the cauliflower mosaic virus (CaMV) and the 3&rsquo;-polyadenylation signal from the nopaline synthase gene (<i>nos</i>) from <i>A. tumefaciens</i>. Expression of the <i>neo</i> gene was under the control of the <i>nos</i> promoter sequence and octopine synthase termination sequence from <i>A. tumefaciens</i>.</p>
