<p style="text-align: justify;">The transgenic Bt cotton was developed by using the biolistic method of transformation system. The DNA transferred from the plasmid to the genome of individual cotton cells (T-DNA), included the <i>cry1Ac</i> and <i>nptII</i> genes. The transformed cotton shoots containing the <i>nptII</i> gene were selected on medium supplemented with kanamycin. A procedure for biolistics method of transformation of cotton is novel and performed using shoot meristem. Plants were regenerated and ultimately plantlets were grown in soil and assayed for insect resistance.</p>
