<p style="text-align: justify;">These transgenic rice lines were produced using a direct DNA transfer system in which DNA sequences encoding the PAT enzyme were introduced into callus tissue of the parental rice varieties M202, in the case of LLRICE06, and Bengal, in the case of LLRICE62. The nucleotide sequence of the native <i>bar</i> gene was modified to improve expression in plant cells, which was regulated by including promoter and transcription-termination and polyadenylation sequences derived from the 35S transcript of cauliflower mosaic virus (CaMV). The <i>bar</i> gene, including these regulatory sequences, was the only novel gene introduced into the parental rice varieties.</p>
