<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">Southern blot analysis of genomic DNA from BXN&trade; cotton demonstrated the presence of one or two sites of integration of the T-DNA within the host genome. There was no evidence of integration of plasmid DNA sequences outside of the T-DNA region.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The nitrilase enzyme was expressed at very low levels in unprocessed whole cottonseed, cottonseed hulls, and cottonseed meal, which are used in livestock feed. It was estimated that the enzyme comprised about 0.0006% of seed protein, which itself makes up a very small proportion of total meal protein. Refined cottonseed oil, which is the major cotton product consumed by humans, did not contain any detectable amounts of nitrilase enzyme. This result was expected as refined oils are generally free of DNA and protein.</p>
