<p style="text-align: justify;"><b>The Inserted DNA</b></p>

<p style="text-align: justify;">Southern blot analysis of genomic DNA from line A2704-12 indicated the incorporation of two copies of the <i>pat</i> gene, inserted in a head-to-tail configuration, and that one copy of the 3&#39; <i>bla</i> sequences and one copy of the 5&#39; <i>bla</i> sequences were integrated between the two <i>pat</i> gene copies.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The PAT enzyme was the only new protein expressed in these transgenic soybean lines. The levels of PAT protein expression were quantitated using enzyme linked immunosorbent assay (ELISA), which in the case of A2704-12 were found to correspond to 0.0031%, 0.0015% and 0.0005% of the total crude protein in samples of forage, hay and seed, respectively. As expected, no PAT protein was detected in refined oil, food grade oil, and crude lecithin. Although PAT enzyme activity could be detected in the hull fraction, no activity was detected in meal fractions prepared from A2704-12.</p>
