<p style="text-align: justify;"><b>Nutritional Data (Livestock Feed Purposes)</b></p>

<p style="text-align: justify;">Data on fatty acid profiles, protein content, amino acid composition, crude fibre, ash, phytate, and moisture content were provided for grain samples from lines IMINTA 1 and IMINTA 4 and the parental variety IRGA 417 grown in two locations in Argentina. Comparisons of these parameters between the herbicide-tolerant lines and conventional variety revealed some statistically significant differences (lower crude fat and oleic acid levels and lower levels of panthothenic acid), but levels were all within the range for conventional rice varieties.</p>

<p style="text-align: justify;"><b>Toxicity and Allergenicity</b></p>

<p style="text-align: justify;">The single amino acid change within the native ALS enzyme was not anticipated to have any effect on the potential toxicity or allergenicity of this protein. The unmodified form of this enzyme does not display amino acid sequence homology with known protein toxins or allergens. The modified ALS protein from IMINTA 1 and IMINTA 4 was shown to be rapidly inactivated by heat (1 minute at 100&deg;C) and equivalent to the parental controls with respect to trypsin degredation. Susceptibility to heat inactivation and sensitivity to proteolytic degradation are not characteristics of known protein toxins or allergens.</p>

<p style="text-align: justify;">Measurements of the concentrations of normally occurring rice antinutritional factors (<i>e.g.</i>, phytic acid and trypsin inhibitor) did not reveal any differences between these herbicide-tolerant lines and the parental rice variety. For all of the lines, the concentrations of trypsin inhibitor were below the threshold of detection.</p>
