<p style="text-align: justify;">Oxy-235 was produced by <i>Agrobacterium</i>-mediated transformation of the <i>B. napus</i> cultivar &#39;Westar&#39; with plasmid pRPA-BL-150a using the EHA 101 strain of <i>A. tumefaciens</i>. Transformed plants were regenerated in tissue culture using bromoxynil as the sole selective agent. The original transformationevent --Westar-Oxy-235-- was used as a parental line in the production of commercial canola varieties marketed under the Navigator&trade; name.<br />
Plasmid pRPA-BL-150a was a double border binary Ti vector containing one copy of the <i>bxn</i> gene (1150 bp) under the control of the CaMV 35S promoter and an enhancer region from the non-translated portion of the RuBisCo SSU gene from maize. The construct also contained the nopaline synthase 3&#39; non-coding regions from <i>A. tumefaciens</i> (plasmid pTi37).</p>
