Monsanto used Bobwhite spring wheat (designated as MON 71900) as the parental variety to produce Roundup Ready® wheat event MON 71800. Event MON 71800 was developed through Agrobacterium-mediated transformation using the double border, binary vector PVTXGT10. The vector consists of the T-DNA segment intended for transformation, as well as the backbone DNA which is not expected to be incorporated into the transformed wheat.<br>The T-DNA contains two cp4 epsps gene cassettes and the left and right T-DNA border sequences. The segment is approximately 7.0 Kb in size. The cp4 epsps coding sequence in both cassettes is derived from Agrobacterium sp. strain CP4 and begins with a leader chloroplast transit peptide coding sequence (ArabTP) derived from the Arabidopsis thaliana epsps gene. The ArabTP transit peptide directs the CP4 EPSPS protein expressed in event MON 71800 to chloroplasts. The ArabTP-cp4 epsps fusion coding sequence in each cassette is followed by the 3' non-translated region of the nopaline synthase gene (nos 3') from Agrobacterium tumefaciens that provides the transcriptional termination signal. The upstream regulatory sequence of the first cassette consists of the 5' region of the rice actin1 gene (Pract1- ract1I) which contains the promoter, transcription start site and the first intron. The upstream regulatory sequence of the second cassette consists of an enhanced 35S promoter from the cauliflower mosaic virus (CMV) (P-e35S) and the intron of the corn heat shock protein gene (hsp70I). The two different promoters were used to drive expression of the gene product in both vegetative and reproductive tissues.