<p style="text-align: justify;">Line A5547-127 was produced via biolistic transformation of soybean with a pUC19 based plasmid containing a modified form the <i>pat</i> gene under the control of promoter and termination sequences derived from the 35S transcript from cauliflower mosaic virus (CaMV). The plasmid was linearized prior to transformation in order to destroy the beta-lactamase (<i>bla</i>) encoding antibiotic resistance marker gene present in the plasmid backbone.</p>

<p style="text-align: justify;">The nucleotide sequence of the <i>pat</i> gene was altered via site-directed mutagenesis in order to reduce the high G:C content (typical for bacterial genes but atypical for plant genes) and to generate plant-preferred codons. These sequence modifications did not result in changes to the predicted amino acid sequence of the PAT enzyme.</p>
