<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">Southern blot analyses of the genomic DNA from H7-1 confirmed the existence of a single intact copy of the CP4 EPSPS expression cassette, including the promoter, terminator, and chloroplast transit peptide sequences. The analyses also confirmed that none of the plasmid backbone sequences were integrated into the genome of H7-1. Further analyses demonstrated the nearly complete integration of the Left Border sequence and the omission of the Right Border sequence.</p>

<p style="text-align: justify;"><b>Genetic Stability of the Trait</b></p>

<p style="text-align: justify;">Southern blot analyses were conducted on genomic DNA from the F2, F3 and F4 generations of the original transformant. The results demonstrated the stable integration of the CP4 EPSPS expression cassette at a single locus in all generations. The results of Mendelian segregation analysis confirmed that the glyphosate-tolerance trait is inherited as a dominant trait.</p>

<p style="text-align: justify;"><b>Expressed material</b></p>

<p style="text-align: justify;">Levels of CP4 EPSPS were quantified in samples of H7-1 processed roots (brei) and leaves (tops) from 6 field trials conducted in Europe. Quantification was achieved using a double-antibody sandwich ELISA (enzyme-linked immunosorbent assay) technique, and results were expressed on a fresh weight basis. The mean level of CP4 EPSPS in leaves was 161 &micro;g/g (range: 112 &ndash; 201 &micro;g/g), and 181 &micro;g/g (145-202 &micro;g/g) in processed roots.</p>

<p style="text-align: justify;">Sugar beet intended for human consumption is generally converted directly to refined white sugar through extensive purification processes. The levels of CP4 EPSPS protein introduced into H7-1 sugar beet were not measured in refined sugar. It was assumed that since refined sugar does not contain detectable levels of protein, there would be virtually no human exposure to CP4 EPSPS. Consequently, there is no anticipated human exposure to this protein as a result of consumption of refined sugar derived from H7-1 sugar beet.</p>
