<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">Southern and PCR analyses of genomic DNA from event T120-7 indicated the presence of a single insertion of the T-DNA region, and no evidence of insertion of sequences outside the T-DNA borders.</p>

<p style="text-align: justify;"><b>Genetic Stability of the Introduced Trait</b></p>

<p style="text-align: justify;">Stable insertion of the T-DNA was demonstrated by comparing the original transformant with four progenies produced either by self-pollination or crosses with nontransgenic sugar beet lines. From Southern blot analyses and trait segregation data, it was concluded that the trait was maintained over multiple generations and inherited in a Mendelian fashion.<br />
<br />
<b>Expressed Material</b></p>

<p style="text-align: justify;">The expression levels of PAT enzyme were quantitated using enzyme linked immunosorbent assay (ELISA) and found to average 137 ng/g (fresh wt tissue; range 74-208 ng/g) in the roots, 966 ng/g (fresh wt tissue; range 732-1176 ng/g) in the tops, and undetectable in the pulp, molasses or refined sugar. The concentrations of NPTII averaged 20 ng/g (fresh wt tissue) in the roots, 44 ng/g (fresh wt tissue) in tops, and undetectable in molasses or refined sugar.</p>
