Soybean transformation event A5547-127 has been<br> genetically engineered to express a single copy of the<br> synthetic pat gene isolated from a soil bacterium<br> Streptomyces viridochromogenes that results in the<br> expression of an enzyme phosphinothricin acetyl<br> transferase (PAT), which confers resistance to glufosinate<br> herbicide.  In addition the glufosinate resistant soybean<br> transformation event A5547-127 also contains partial<br> copies of the bacterial selection marker gene bla that is not expressed.   <br> The soybean transformation event A5547-127 has been<br> transformed with a vector pB2/35SAcK (has the same<br> backbone structure derived from pUC plasmids) that has <br> only two functional genes, viz the phosphinothricin acetyl<br> transferase pat, and bla coding for ampicillin resistance<br> via the enzyme  -lactamase.  Only the pat is fully intact<br> and functional in the plants whereas the bla gene is not. <br> The pat gene is under the influence of 35S cauliflower<br> mosaic virus promoter and terminator.  Glufosinate resistant soybean transformation event A5547-<br> 127 is significantly similar to the antecedent organisms<br> described in the AgrEvo petition 96-068-01p except that it<br> was developed by a new transformation event using the same<br> biolistic particle bombardment technique.  A5547-127 is<br> tolerant to the herbicide glufosinate and exhibits the<br> identical agronomic characteristics as the antecedent organisms.  <br> A5547-127 contains broken bla gene which is not expressed<br> and all the regulatory sequences used to express the pat<br> gene come from the cauliflower mosaic virus, a well known<br> plant pest.  The vector used for transformation also has<br> the same backbone drawn from pUC family of vectors.  In<br> addition, A5547-127 contains a single copy of the pat gene<br> where as the antecedent organisms had 3 copies of the<br> same.   There is no gus gene in A5547-127.