<p style="text-align: justify;">The BXN&trade; cotton line was created by <i>Agrobacterium</i>-mediated transformation in which the transfer-DNA (T-DNA) contained the gene encoding the enzyme nitrilase from the soil bacterium <i>Klebsiella pneumoniae</i> subsp. ozaenae. For expression in plants, all but 11 bp of the 5&rsquo; and 96 bp of the 3&rsquo; non-translated regions of the gene were used. In addition, the T-DNA contained sequences encoding the enzyme neomycin phosphotransferase II (NPTII) from the Tn5 transposon of <i>E. coli</i>, strain K12.</p>
