<p style="text-align: justify;"><b>Nutritional Data</b></p>

<p style="text-align: justify;">Samples of hay, forage, seed, hulls, and toasted and non-toasted defatted soy meal from transgenic soybean were subjected to proximate analyses (moisture, crude protein, crude fat, ash, acid detergent fibre, neutral detergent fibre, carbohydrate). As well, seed samples were subjected to fatty acid analysis, amino acid analysis, and analyses of minerals (calcium, phosphorous, and potassium). Except in one case, there were no statistaically significant differences between the values determined for transgenic plant samples and corresponding samples obtained from non-transgenic control plants. Statistical differences were seen in the levels of some amino acids between A2704-12 soybeans and non-transgenic soybean, but that the values were still within the normal range reported by the USDA for soybean.</p>

<p style="text-align: justify;"><b>Toxicology</b></p>

<p style="text-align: justify;">Samples of seed were analyzed for stachyose, raffinose, and phytic acid, and additionally for trypsin inhibitor and lectins in seed. The concentrations of phytoestrogens, such as daidzein, genistein, and glycitein, were determined for samples of seed, and toasted and non-toasted soy meal. The levels of these compounds were not statistically different between samples from non-transgenic and transgenic soybeans.</p>

<p style="text-align: justify;"><b>Allergenicity</b></p>

<p style="text-align: justify;">The low potential for allergenicity of the PAT protein has previously been established through amino acid sequence comparisons with known protein allergens and digestability studies using simulated gastric and intestinal fluids. Additionally, soybean seed extracts from line A2704-12 and from non-transgenic control plants were screened against a panel of sera from 16 soy-allergic individuals using the radioallergosorbent test (RAST). The results of this study did not reveal any qualitative or quantitative difference in endogenous soybean allergen content between transgenic and non-transgenic soybean.</p>
