<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">Analysis of MON802 genomic DNA by Southern blotting indicated that there were two sites of integration of the introduced DNA, with the first site containing a single full length copy of the <i>cry1Ab</i>, CP4 EPSPS, and <i>neo</i> genes, two full length copies of the <i>gox</i> gene, and two partial copies of the <i>cry1Ab</i> gene. The second site contained only the <i>gox</i> and <i>neo</i> gene sequences.</p>

<p style="text-align: justify;"><b>Genetic Stability of the Introduced Trait</b></p>

<p style="text-align: justify;">Data from successive generations of backcrosses indicated that both sites of integration segregated as a single locus and according to the rules of Mendelian inheritance.</p>

<p style="text-align: justify;">The traits have remained stable for six generations as shown by assays for Cry1Ab, and herbicide tolerance.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The expression of the Cry1Ab protein was quantitated by enzyme linked immunosorbent assay (ELISA) of tissue samples obtained from plants grown at a number of field trial locations. The average Cry1Ab concentrations were: 9.55 &micro;g/g fresh weight (fwt) from leaf tissue (6 locations); 1.35 &micro;g/g fwt from whole plant matter (2 locations); and 3.2 &micro;g/g fwt from grain (6 locations).</p>

<p style="text-align: justify;">Similarly, expressed CP4 EPSPS protein concentrations were: 26.99 &micro;g/g fwt from leaf tissue; 1.85 &micro;g/g fwt from whole plant tissue; and 2.27 &micro;g/g fwt from grain. The average concentrations of GOX protein were: 10.18 &micro;g/g fwt from leaf tissue; 1.68 &micro;g/g fwt from whole plant tissue; and undetectable in grain.</p>

<p style="text-align: justify;">The Cry1Ab protein was shown to degrade readily in the environment. The plant expressed protein had DT50 and DT90 values (time to degrade to 50% and 90% of the original bioactivity) of 1.6 and 15 days respectively.</p>
