<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">Southern blot analyses of genomic DNA from line 55-1 verified that it contained the PRSV CP gene, intact copies of two plant-expressible marker genes encoding NPTII and GUS, respectively, and a partial copy of the tetracycline resistance marker gene. Genomic DNA did not hybridize with probes to the gentamycin marker genes or to the origin of bacterial replication (Ori V/Tet) region. The partial tetracycline resistance gene was not expressed in plants, due both to the fact that the gene was incomplete and under the regulatory control of a bacterial promoter.</p>

<p style="text-align: justify;">Southern blot analyses indicated that line 63-1 contained intact, functional genes encoding the PRSV CP and NPTII, and did not contain the GUS encoding gene. Genomic hybridization with probes to the gentamycin resistance gene and the Ori T/Tet region indicated that either all or part of the genes for gentamycin and tetracycline resistance had integrated into the papaya genome. However, these genes were not functional since their bacterial promoters cannot drive expression of these genes in plants.</p>
