<p style="text-align: justify;">Maize line MON80100 was produced by biolistic transformation of maize genotype B73 with a mixture of DNA from two plasmids. One plasmid contained a copy of the <i>cry1Ab</i> gene from <i>B. thuriengensis</i> subsp. kurstaki (B.t.k.). Constitutive expression of the <i>cry1Ab</i> gene was controlled by inclusion of sequences from the 35S promoter of cauliflower mosaic virus (CaMV), the HSP70 intron and the 3&#39;-polyadenylation signal of the nopaline synthase (<i>nos</i>) gene from <i>Agrobacterium tumefaciens</i>. The second plasmid contained open reading frames for two selectable marker genes, the CP4 EPSPS enzyme from the common soil bacterium, <i>Agrobacterium tumefaciens</i> sp. CP4, and glyphosate oxidoreductase (<i>goxv247</i>) from <i>Ochrobactrum anthropi</i> sp. strain LBAA. Constitutive expression of these genes in plant cells was under the control of the CaMV 35S promoter and <i>nos</i> terminator. Post-translational targeting of the CP4 EPSPS and glyphosate oxidoreductase enzymes to the chloroplast was accomplished by fusion of the 5&#39;-terminal coding sequences with the chloroplast transit peptide DNA sequences from the <i>Arabidopsis thaliana</i> EPSPS and ribulose-1,5-bisphosphate carboxylase encoding genes, respectively. Both plasmids also contained sequences encoding the enzyme neomycin phosphotransferase II (NPTII) from the Tn5 transposon of <i>Escherichia coli</i>, strain K12. Expression of the <i>neo</i> gene was regulated by a bacterial promoter and was used as a selectable trait to screen transformed <i>E. coli</i> for the presence of the plasmid vector. The expression of CP4 EPSPS and glyphosate oxidoreductase activity were used as selectable markers to identify transformed plants. Apart from the sequences encoding Cry1Ab, CP4 EPSPS and glyphosate oxidoreductase, no other plant-translatable DNA sequences were introduced into the plant genome.</p>
