<p style="text-align: justify;">This bioengineered tomato was produced by <i>Agrobacterium</i>-mediated transformation of tomato (<i>Lycopersicon esculentum</i>) in which the transfer-DNA (T-DNA) region of the bacterial tumour inducing (Ti) plasmid was modified to contain DNA sequences encoding an &ldquo;antisense&rdquo; PG gene construct and the NPTII encoding <i>neo</i> gene from <i>E. coli</i> K12. During transformation, the T-DNA portion of the plasmid was transferred into the plant cells and stably integrated into the plant&#39;s genome.</p>

<p style="text-align: justify;">The antisense PG gene was under the regulatory control of a single copy of the 35S promoter from the cauliflower mosaic virus (CaMV), or two tandem copies of the 35S promoter. The terminator sequences were from the <i>tml</i> gene and the transcript 7 gene from the octopine-type Ti plasmid pTiA6 from <i>A. tumefaciens</i>. Expression of the <i>neo</i> gene was under the control of the 5&#39; promoter and 3&#39; terminator sequences from the mannopine synthase gene derived from <i>A. tumefaciens</i>.</p>
