<p><b style="color: rgb(89, 89, 89); font-family: Verdana, Helvetica, Arial, 'Sans Serif'; font-size: 11px; line-height: 14px; text-align: justify;">Molecular Characterization:</b><br style="color: rgb(89, 89, 89); font-family: Verdana, Helvetica, Arial, 'Sans Serif'; font-size: 11px; line-height: 14px; text-align: justify;" /><br><span style="color: rgb(89, 89, 89); font-family: Verdana, Helvetica, Arial, 'Sans Serif'; font-size: 11px; line-height: 14px; text-align: justify;">Detailed Southern blot analysis of 4114 maize genomic DNA confirmed that the introduced DNA (T-DNA) was incorporated intact into a single site within the maize genome, without truncation or deletion of nucleotide sequences within any of the gene expression cassettes. Based on a combination of genotypic and phenotypic testing, it was determined that the introduced&nbsp;</span><i style="color: rgb(89, 89, 89); font-family: Verdana, Helvetica, Arial, 'Sans Serif'; font-size: 11px; line-height: 14px; text-align: justify;">cry1F, cry34Ab1, cry35Ab1</i><span style="color: rgb(89, 89, 89); font-family: Verdana, Helvetica, Arial, 'Sans Serif'; font-size: 11px; line-height: 14px; text-align: justify;">, and&nbsp;</span><i style="color: rgb(89, 89, 89); font-family: Verdana, Helvetica, Arial, 'Sans Serif'; font-size: 11px; line-height: 14px; text-align: justify;">pat</i><span style="color: rgb(89, 89, 89); font-family: Verdana, Helvetica, Arial, 'Sans Serif'; font-size: 11px; line-height: 14px; text-align: justify;">&nbsp;genes were stably inherited across multiple generations, and segregated as a single genetic locus in 4114 maize according to Mendelian rules of inheritance.&nbsp;</span><br style="color: rgb(89, 89, 89); font-family: Verdana, Helvetica, Arial, 'Sans Serif'; font-size: 11px; line-height: 14px; text-align: justify;" /><br><b style="color: rgb(89, 89, 89); font-family: Verdana, Helvetica, Arial, 'Sans Serif'; font-size: 11px; line-height: 14px; text-align: justify;">Expressed Material:</b><br><span style="color: rgb(89, 89, 89); font-family: Verdana, Helvetica, Arial, 'Sans Serif'; font-size: 11px; line-height: 14px; text-align: justify;">The Cry1F, Cry34Ab1, Cry35Ab1, and PAT proteins present in 4114 maize are predicted, based on the method of genetic modification and nucleotide sequencing of the introduced DNA, to have the same amino acid sequence as the corresponding proteins in events 1507 and 59122, and by extension, the conventional breeding stack of 1507 x 59122 maize. Protein equivalence was further demonstrated via western immunoblot analysis comparing the molecular weight and immunoreactivity of the Cry1F, Cry34Ab1, Cry35Ab1, and PAT proteins produced either in 4114 maize or in 1507 x 59122 maize. Therefore, previous safety assessments of the Cry1F, Cry34Ab1, Cry35Ab1, and PAT proteins conducted during the reviews of 1507 and 59122 maize are also applicable for 4114 maize. In most cases, the concentrations of Cry1F, Cry34Ab1, Cry35Ab1, and PAT proteins in 4114 maize tissue samples were similar to, or lower than, corresponding concentrations of these proteins in tissue samples from 1507 x 59122 maize. Thus, evaluations of potential environmental exposure to Cry1F, Cry34Ab1, and Cry35Ab1 proteins conducted during the previous assessments of 1507 and 59122 maize are also informative for 4114 maize. Additionally, it is unlikely that any of the differences in the concentrations of Cry1F, Cry34Ab1, or Cry35Ab1 proteins between 4114 and 1507 x 59122 maize are biologically meaningful with respect to potential impact on non-target organisms.</span></p><br>