<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">Southern blot analyses of genomic DNA from line 1345-4 demonstrated the insertion of three copies of the T-DNA at a single insertion site. There was no incorporation of translatable plasmid DNA sequences outside of the T-DNA region.</p>

<p style="text-align: justify;"><b>Genetic Stability of the Introduced Trait</b></p>

<p style="text-align: justify;">It was determined that tomato line 1345-4 was stable and that the introduced genes segregated as a single locus in a Mendelian fashion.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The only novel protein expressed in tomato line 1345-4 was the enzyme NPTII, which was expressed at very low levels ranging from 0.72 to 2.36 &micro;g/mg fresh weight of tomato. While transcription of the truncated ACC synthase gene was detected in transgenic tomato tissues, the partial gene did not express a protein product.</p>
