<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">Southern blot analysis of genomic DNA from DLL25 demonstrated that the <i>bar</i> gene was integrated at a single site of insertion into maize line DLL25. Further analysis demonstrated that DLL25 contained a partially integrated antiobiotic resistant marker (<i>bla</i> gene), which was not expressed.</p>

<p style="text-align: justify;"><b>Genetic Stability of the Introduced Trait</b></p>

<p style="text-align: justify;">Segregation analysis over twelve generations indicated that the introduced <i>bar</i> gene was stably inherited and that it segregated as a single locus, consistent with the Southern blot data demonstrating a single site of insertion. Stability of expression was demonstrated to be consistent across several hybrids derived from DLL25, across several generations.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The only newly expressed protein in DLL25 is PAT protein, which was expressed in leaves, roots, stalk, tassel, cob, husk, kernels, silk and pollen. Highest levels were found in leaves (1.0 - 4.6 &micro;g/g fresh weight tissue, corresponding to 0.1 - 0.46% of total soluble protein), and no PAT protein was detected in either silks, pollen or mature seeds.</p>
