<p style="text-align: justify;"><b>Dietary Exposure</b></p>

<p style="text-align: justify;">Grain from maize line MON832 was intended mainly for use in animal feed. The major human food uses for maize are extensively processed starch and oil fractions prepared by wet or dry milling procedures and products include corn syrup and corn oil, neither containing protein. Human exposure to the modified protein from whole grain corn in the diet was considered to be very low due both to its low abundance in the protein fraction of the grain and to the proportionately low percentage of protein in the kernel, compared with the major starch component. Overall, the dietary exposure of consumers in the United States and Canada to grain from MON832 maize was anticipated to be the same as for other lines of commercially available field corn.</p>

<p style="text-align: justify;"><b>Nutritional Data</b></p>

<p style="text-align: justify;">The major components of maize grain and forage from MON832 were analysed on plant material harvested from field trials. Compositional data for protein, fat, ash, carbohydrates, calories, moisture, amino acids, and fatty acids for line MON832 grain were comparable to the data from the control line and within published ranges for commercial hybrids. Similar promximate analysis, including acid detergent fibre and neutral detergent fibre analyses, were performed on forage for line MON832 and an appropriate control. Based on these compositional data, it was concluded that there were no significant differences between the forage from line MON832 and the control line.</p>

<p style="text-align: justify;"><b>Toxicity</b></p>

<p style="text-align: justify;">The low potential for toxicity of transgenic maize line MON832 was demonstrated by examining the amino aid sequence homology, protein characterization, digestive fate studies in simulated gastric and intestinal fluids and acute oral toxicity in mice. Using sophisticated computerized search capabilities, the evidence showed that the CP4 EPSPS and GOX proteins did not show meaningful amino acid sequence homology when compared to known allergens or protein toxins. These studies support the safety of the proteins and are consistent with the history of safe presence and ubiquitous distribution of <i>Agrobacterium</i> and <i>Achromobacter</i> spp. in the soil. An acute mouse toxicity study further indicated that there were no toxic effects caused by CP4 EPSPS or GOX proteins administered by oral gavage, as measured by the absence of treatment related adverse effects in mice.</p>

<p style="text-align: justify;"><b>Allergenicity</b></p>

<p style="text-align: justify;">The CP4 and GOX proteins are not likely to be toxic or allergenic. The amino acid sequences of both proteins were compared with known toxins and allergens did not show meaningful amino acid sequence homology. In addition, the potential for allergenicity was assessed based upon the characteristics of known food allergens (stability to digestion, stability to processing). Unlike known protein allergens, studies have shown that the CP4 and GOX proteins are rapidly degraded and their enzymatic activity lost under conditions that simulate mammalian digestion.</p>
