<p style="text-align: justify;">Canola line GT200 was produced by <i>Agrobacterium</i>-mediated transformation of the <i>B. napus</i> cultivar &#39;Westar&#39;. The T-DNA region of the Ti plasmid was &quot;disarmed&quot; by removal of <i>vir</i>A genes, normally associated with the pathogenicity and disease-causing properties of <i>A. tumefaciens</i>, and replaced with the genes coding for glyphosate tolerance. During transformation, the T-DNA portion of the plasmid was transferred into the plant cells and stably integrated into the plant&#39;s genome.</p>

<p style="text-align: justify;">GT200 was produced using a plasmid that contained the CP4 EPSPS gene and the <i>gox</i> gene. Both genes were controlled by the same constitutive promoter and fused to a chloroplast transit peptide sequence to allow targeting of the newly translated CP4 EPSPS and GOX enzymes into the chloroplast, the site of aromatic amino acid biosynthesis and endogenous EPSPS and GOX activity.</p>

<p style="text-align: justify;">There was no incorporation of translatable plasmid DNA sequences outside of the T-DNA region and no antibiotic resistance marker genes were introduced into GT200.</p>
