<p><span style="color: rgb(89, 89, 89); font-family: Verdana, Helvetica, Arial, 'Sans Serif'; font-size: 11px; line-height: 14px; text-align: justify;">Agronomic and phenotypic evaluations of 4114 maize were conducted in order to support a conclusion of &ldquo;familiarity&rdquo; based on the similarity of phenotypic characteristics with conventional and/or control maize. The phenotypic evaluations were based on laboratory experiments, to examine seed dormancy and germination, and replicated, multi-location field trials to collect data on representative characteristics that influence reproductive and survival biology. In each of these assessments, 4114 maize was compared to a near?isogenic control (i.e.,&nbsp;</span><i style="color: rgb(89, 89, 89); font-family: Verdana, Helvetica, Arial, 'Sans Serif'; font-size: 11px; line-height: 14px; text-align: justify;">ca</i><span style="color: rgb(89, 89, 89); font-family: Verdana, Helvetica, Arial, 'Sans Serif'; font-size: 11px; line-height: 14px; text-align: justify;">. 98 percent similar) that did not go through the transformation process, and/or to commercial maize hybrids.</span><br style="color: rgb(89, 89, 89); font-family: Verdana, Helvetica, Arial, 'Sans Serif'; font-size: 11px; line-height: 14px; text-align: justify;" /><br><span style="color: rgb(89, 89, 89); font-family: Verdana, Helvetica, Arial, 'Sans Serif'; font-size: 11px; line-height: 14px; text-align: justify;">Germination rates of 4114 maize seed under warm, cold, and diurnal conditions were comparable to those of control maize under corresponding conditions. No potentially dormant seed were identified using a standard tetrazolium chloride test, which was applied to any non-germinated seed that were classified as hard or imbibed. Maize line 4114 can therefore be considered comparable to conventional maize with respect to seed germination and potential dormancy.</span><br><span style="color: rgb(89, 89, 89); font-family: Verdana, Helvetica, Arial, 'Sans Serif'; font-size: 11px; line-height: 14px; text-align: justify;">Comparisons of emergence and vegetative growth parameters between 4114 and control maize were based on assessments of early plant population, seedling vigour, plant height, ear height, stalk lodging, root lodging, final plant population, and stay green. There were no statistically significant differences in any of these parameters between 4114 and control maize.</span><br><span style="color: rgb(89, 89, 89); font-family: Verdana, Helvetica, Arial, 'Sans Serif'; font-size: 11px; line-height: 14px; text-align: justify;">Potential changes to the reproductive characteristics of 4114 maize were assessed through a combination of laboratory evaluations of seed germination and dormancy (previously described); evaluation of pollen viability, as correlated to pollen shape and colour; and collection of relevant field data on time to silking, time to pollen shed, and yield. In addition, visual estimates of foliar disease incidence and insect damage were collected for both 4114 and control maize at each location. There were no statistically significant differences in any of the parameters related to reproductive biology or disease and pest susceptibility between 4114 and control maize.</span></p><br>