<p style="text-align: justify;">The transgenic carnation lines 959A, 988A, 1226A, 1351A, 1363A, and 1400A were produced by <i>Agrobacterium</i>-mediated transformation of carnation plants (<i>Dianthus caryophyllus</i> L., cultivar &lsquo;White Unesco&rsquo;) in which the transfer-DNA (T-DNA) region of the bacterial Ti plasmid was modified to contain the DFR and F3&rsquo;, 5&rsquo;H encoding genes as well as the surB (ALS encoding) gene from a chlorsulfuron tolerant line of tobacco.</p>

<p style="text-align: justify;">Expression of the DFR encoding gene was under the control of its own promoter and terminator sequences. Expression of the F3&rsquo;, 5&rsquo;H encoding gene was under the control of the petal-specific chalcone synthase (CHS) promoter and the D8 terminator, both derived from <i>Petunia hybrida</i>.</p>

<p style="text-align: justify;">The <i>surB</i> gene was used as a selectable marker to identify transformed plants during tissue culture regeneration. Expression of the ALS enzyme was regulated using the CaMV 35S promoter and the surB terminator derived from tobacco (<i>Nicotiana tabacum</i>).</p>
