<p style="text-align: justify;"><span style="font-size: 12px;"><span style="font-family: arial,helvetica,sans-serif;">The transgenic Atlantic and Superior potato lines were created through two separate <i>Agrobacterium</i>-mediated transformation events in which the transfer DNA (T-DNA) contained the gene encoding the Cry3A protein from <i>B. thuringiensis</i> subsp. Tenebrionis. In addition, the T-DNA contained sequences encoding the enzyme neomycin phosphotransferase II (NPTII). The expression of NPTII was used as a selectable trait for screening transformed plants for the presence of the <i>cry3A</i> gene. Due to segregation effects, the NPTII encoding gene was not present in Superior cultivar SPBT02-5 submitted for commercial release. Additional DNA outside of the T-DNA border sequences was incorporated into the genome of Atlantic lines ATBT04-27 and ATBT04-36. These lines also contain the <i>aad</i> gene, which encodes the enzyme 3&#39;’(9)-O-aminoglycoside adenylyltransferase that confers bacterial resistance to spectinomycin and streptomycin. The <i>aad</i> gene was not expressed in plant tissue, but was present on the Ti plasmid as a selectable trait for screening bacterial colonies for the presence of the plasmid vector. The data from at least 3 generations of vegetative propagation demonstrated the stability of the novel trait.</span></span></p><br>