<p style="text-align: justify;">DAS-59122-7 maize was produced by <i>Agrobacterium</i>-mediated transformation of the hybrid maize line Hi-II. The T-DNA segment of the vector plasmid PHP17662 contained sequences corresponding to synthetic forms of the <i>cry</i>34Ab1 and <i>cry</i>35Ab1 genes from <i>Bacillus thuringiensis</i> strain PS149B1, and the phosphinothricin N-acetyltransferase (PAT) encoding <i>pat</i> gene from <i>Streptomyces viridochromogenes</i>. The <i>cry</i>34Ab1 and <i>cry</i>35Ab1 gene sequences were modified to contain codons to optimize their expression in maize. Transcription of the <i>cry</i>34Ab1 gene was directed by the promoter, intron and 5&#39; untranslated region (UTR) sequences from the maize ubiquitin gene. Terminator sequences were derived from <i>Solanum tuberosum</i> proteinase inhibitor II (PINII). The expression of the <i>cry</i>35Ab1 gene was regulated by the root-preferred promoter from the <i>Triticum aestivum</i> peroxidase gene. Terminator sequences for the <i>cry</i>35Ab1 gene were identical to those for <i>cry</i>34Ab1. The expression of the <i>pat</i> gene was regulated by the 35S promoter from the Cauliflower Mosaic Virus (CaMV). Terminator sequences were derived from the CaMV 35S terminator.</p>
