<p style="text-align: justify;">Soybean line MON89788 was produced by <i>Agrobacterium tumefaciens</i>-mediated transformation of meristematic tissue from soybean cultivar A3244. The plasmid PV-GMGOX20 used for transformation contained the <i>aroA</i> (<i>epsps</i>) gene from <i>A tumefaciens</i> strain CP4 encoding the CP4 EPSPS protein for glyphosate tolerance. Expression of the CP4 EPSPS gene was regulated by a chimeric promoter combining the enhancer sequences from the 35S promoter of the Figwort Mosaic Virus and the promoter from the <i>Tsf1</i> gene from <i>Arabidopsis thaliana</i>, which codes for the elongation factor, EF-1 alpha. A chloroplast transit peptide (CTP2) coding sequence from the <i>ShkG</i> gene of <i>A. thaliana</i> facilitated the translocation of newly translated EPSP synthase into chloroplasts, the site of aromatic amino acid biosynthesis and glyphosate site of action. The 3&rsquo; nontranslated sequence from the ribulose-1, 5-bisphosphate carboxylase small subunit (<i>RbcS2</i>) <i>E9</i> gene of pea (<i>Pisum sativum</i>) provided the transcriptional termination sequences. This cassette, coding for the expression of the CP4 EPSPS enzyme, was contained between the Right and Left Border sequences of the T-DNA region in PV-GMGOX20.</p>
