<p style="text-align: justify;">Maize line MON832 was produced by biolistic transformation of embryogenic maize cells with a mixture of DNA from two plasmids, PV-ZMBK07 and PV-ZMGT10. Plasmid PV-ZMBK07 contained the synthetic <i>cr1Ab</i> gene regulated by the enhanced duplicated cauliflower mosaic virus 35S promoter (CaMV E35S) and the maize hsp70 heat shock protein intron. The polyadenylation signal was from the <i>Agrobacterium tumefaciens</i> nopaline synthase (<i>nos</i>) gene.</p>

<p style="text-align: justify;">The second plasmid, PV-AMGT10, contained genes encoding the CP4 EPSPS enzyme from the common soil bacterium, <i>A. tumefaciens</i> sp. CP4, and glyphosate oxidoreductase (<i>goxv247</i>) from <i>Ochrobactrum anthropi</i>. Constitutive expression of these genes in plant cells was under the control of the CaMV E35S promoter, the hsp70 intron, and <i>nos</i> 3&#39; terminator. Post-translational targeting of the CP4 EPSPS and glyphosate oxidoreductase enzymes to the chloroplast was accomplished by fusion of the 5&#39;-terminal coding sequences with the chloroplast transit peptide DNA sequences from the <i>Arabidopsis thaliana</i> EPSPS gene (CTP2) and from <i>A. thaliana</i> SSU1A gene (CTP1), respectively. Plasmid PV-ZMGT10 also contained sequences from the <i>lac</i>Z operon, ori-pUC and the <i>neo</i> gene. The <i>neo</i> gene on both plasmids was included as a selectable marker to identify bacteria transformed with recombinant plasmid DNAs. Expression of the <i>neo</i> gene was regulated by a bacterial promoter and therefore is not functional in plants.</p>
