<p style="text-align: justify;">DP356043 was produced by microprojectile-mediated bombardment of embryogenic somatic cell cultures, derived from explants from small, immature soybean seeds of the cultivar, Jack. The DNA was derived from the plasmid PHP20163, which contained two expression cassettes and an antibiotic (hygromycin) resistance marker gene. From this plasmid, a linear fragment was excised, referred to as PHP20163A transformation fragment, and used for transformation by adsorption to gold microparticles. The PHP20163A transformation fragment contained two expression cassettes, the <i>gat4601</i> and the <i>gm-hra</i> gene cassettes, but does not contain the antibiotic resistance marker gene.</p>

<p style="text-align: justify;">The <i>gat4601</i> gene cassette contains the coding sequence of the <i>gat4601</i> gene, which encodes the GAT4601 protein. It is a synthetic gene derived from three <i>gat</i> genes from <i>Bacillus licheniformis</i>. <i>In planta</i> expression of the <i>gat4601</i> gene is under the control of two regulatory elements, the SCP1 promoter and the TMV omega 5&#39;-UTR. The SCP1 promoter is a synthetic constitutive promoter containing a portion of the CaMV 35S promoter and the Rsyn7-Syn II Core synthetic consensus promoter. The TMV omega 5&#39;-UTR is the omega 5&#39; untranslated leader of the Tobacco Mosaic Virus. The SCP1 promoter drives the transcription of the <i>gat4601</i> gene. The TMV omega 5&#39;-UTR, which is located downstream from the SCP1 promoter, enhances translation. Termination of transcription of the <i>gat4601</i> gene is under the control of the <i>pin</i>II terminator, the 3&#39; terminator sequence from the proteinase inhibitor II gene of <i>Solanum tuberosum</i>.</p>

<p style="text-align: justify;">The <i>gm-hra</i> gene cassette contains the coding sequence of the <i>gm-hra</i> gene, which encodes the GM-HRA protein. It is a modified version of the endogenous soybean acetolactate synthase gene (<i>gm-als</i>), which encodes the GM-ALS I protein. Compared to the GM-ALS I protein, the GMHRA protein sequence contains two amino acid substitutions important for tolerance to the ALS inhibiting class of herbicides, and five additional N-terminal amino acids derived from the translation of 15 nucleotides of the <i>gm-als</i> 5&#39; untranslated region. <i>In planta</i> expression of the <i>gm-hra</i> gene is controlled by the promoter derived from the S-adenosyl-L-methionine synthetase (SAMS) gene from soybean, and an intron that interrupts the SAMS 5&#39; untranslated region (5&#39;-UTR). Termination of transcription of the <i>gm-hra</i> gene is under the control of the native soybean acetolactate synthase terminator (<i>gm-als</i> terminator).</p>
