<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">A single amino acid substitution is responsible for the imadazolinone tolerance mutation in the AHAS gene. The substitution is sufficient to alter the binding site for imadazolinones, resulting in a lack of inhibition of the AHAS enzyme.</p>

<p style="text-align: justify;"><b>Genetic Stability of the Introduced Trait</b></p>

<p style="text-align: justify;">The segregation of herbicide tolerance in crosses with AP602CL is consistent with the inheritance of a single semi- or co-dominant allele. AP602CL segregated in a 3:1 ratio ( herbicide resistant: susceptibility to herbicide).</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The modified AHAS enzyme lacks a binding site for imadazolinone herbicides. This prevents the inhibition of the activity of this enzyme by the herbicide. AHAS is active in the production of valine, leucine and isoleucine and inhibition of this activity results in a decrease in protein production and eventual plant death. The modified AHAS has no significant difference in its activity in producing valine, leucine or isoleucine and the protein components of AP602CL are not substantially different from that of its parent line, Gunner.</p>
