<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">Southern blot analysis of genomic DNA from MON832 confirmed that sequences from plasmid PV-ZMBK07, containing the <i>cry1Ab</i> gene, were not incorporated but that the CP4 EPSPS encoding sequences along with three copies of the <i>gox</i> gene and two NPTII/ori-pUC sequences (one complete and one rearranged) were integrated as a single 16 kb insert.</p>

<p style="text-align: justify;"><b>Genetic Stability of the Introduced Trait</b></p>

<p style="text-align: justify;">Segregation and stability data were consistent with a single site of insertion into the genomic DNA of line MON832.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The CP4 EPSPS and GOX enzymes were constitutively expressed in tissues throughout the plant and at all plant growth stages. Enzyme linked immunosorben assay (ELISA) was used to quantitate the expression levels of CP4 EPSPS and GOX in the leaves, forage, and kernels of plants grown in field trials. Average amounts were as follows: in leaves 49.61 EPSPS &micro;g/g fresh weight (fwt) and 4.92 GOX &micro;g/g fwt; in forage 21.32 EPSPS &micro;g/g fwt and 3.32 GOX &micro;g/g fwt; and in kernels 5.83 EPSPS &micro;g/g fwt and 1.97 GOX &micro;g/g fwt.</p>

<p style="text-align: justify;">Western immunoblot analysis was used to confirm that NPT II was not detectable in MON832, which was predicted since expression of the <i>neo</i> gene was regulated by a bacterial promoter known not to be active in plants.</p>
