<p style="text-align: justify;">Sugar beet line GTSB77 was produced by <i>Agrobacterium</i>-mediated transformation of the proprietary cytoplasmic male sterile sugar beet line A1012 with plasmid PV-BVGT03. The T-DNA region of the <i>Agrobacterium</i> Ti plasmid used to develop line GTSB77 contained sequences encoding CP4 EPSPS, glyphosate oxidase (GOX), neomycin phosphotransferase II (NPTII), and GUS.</p>

<p style="text-align: justify;">Associated with the CP4 EPSPS encoding gene were promoter sequences derived from the 35S transcript of figwort mosaic virus (FMV), sequences encoding the chloroplast transit peptide (CTP2) from the <i>Arabidopsis thaliana</i> EPSPS gene, and transcription termination and polyadenylation signal sequences from the ribulose-1,5-bisphosphate carboxylase small subunit (rbcS; rubisco) encoding gene from pea (<i>Pisum sativum</i>).</p>

<p style="text-align: justify;">Expression of the GUS encoding <i>uidA</i> gene was regulated using the enhanced 35S promoter from cauliflower mosaic virus (CaMV) and the transcription termination sequences from the 3&rsquo; region of the pea rbcS encoding gene. The regulatory sequences linked to the <i>Ochrobactrum anthropi</i> GOX encoding gene included the FMV 35S promoter, CTP1 signal sequence from the small subunit 1A of rubisco from <i>A. thaliana</i>, and 3&rsquo; termination sequences from the <i>A. tumefaciens</i> nopaline synthase gene.</p>
