<p style="text-align: justify;">Canola line HCN28 was produced by <i>Agrobacterium</i>-mediated transformation of <i>B. napus</i> cultivar &#39;AC Excel&#39;. The T-DNA region of the Ti plasmid was &quot;disarmed&quot; by removal of <i>vir</i>A genes, normally associated with the pathogenicity and disease-causing properties of <i>A. tumefaciens</i>, and replaced with the gene coding for glufosinate ammonium tolerance. During transformation, the T-DNA portion of the plasmid was transferred into the plant cells and stably integrated into the plant&#39;s genome.</p>

<p style="text-align: justify;">The <i>pat</i> gene used in transformation event T45 is a synthetic version of the gene isolated from <i>Streptomyces viridochromogenes</i>, strain Tu 494. The nucleotide sequence was modified to provide codons preferred by plants without changing the amino acid sequence of the enzyme. Expression of the <i>pat</i> gene was regulated by including promoter and terminator sequences from the 35S transcript of cauliflower mosaic virus (CaMV).</p>

<p style="text-align: justify;">The original transformant (T45) was backcrossed twice with <i>B. napus</i> line AC Excel. HCN28 was developed from the resultant BC2 using the single seed descent method.</p>
