<p style="text-align: justify;"><b>The Inserted DNA</b></p>

<p style="text-align: justify;">Southern blot analysis of genomic DNA from line A5547-127 indicated the incorporation of a single copy of the <i>pat</i> gene flanked by the 5&#39; and 3&#39; partial sequences of the transected <i>bla</i> gene.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The PAT enzyme was the only new protein expressed in these transgenic soybean lines. The levels of PAT protein expression were quantitated using enzyme linked immunosorbent assay (ELISA)and were found to correspond to 0.0174%, 0.0034% and 0.0031% of the total crude protein in samples of forage, hay and seed, respectively. As expected, no PAT protein was detected in refined oil, food grade oil, and crude lecithin.</p>
