<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">Southern blot analysis of genomic DNA from MS3 demonstrated the integration of three copies of the DNA insert containing the barnase and <i>bar</i> genes at a single insertion site.</p>

<p style="text-align: justify;"><b>Genetic Stability of the Introduced Trait</b></p>

<p style="text-align: justify;">The stability of the inserted DNA in the maize line MS3 was evaluated by segregation analyses over at least 6 generations demonstrating that the introduced trait segregated as a single dominant genetic locus and followed Mendelian rules of inheritance.hat showed over several generations. Comparisons between the original transgenic plant and plants from the fourth and sixth generations showed no differences in the gene integration pattern.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The barnase RNAse was detected only in early stages of development of the tapetum cell layer of anthers. It was not detected in the other plant tissues tested: leaves, immature kernels, roots, dry and germinating seeds.<br />
Expression levels of PAT were estimated through the quantification of RNA transcripts: 0.05 pg/&micro;g of RNA was found in leaves and immature kernels, while none was detected in roots, dry and germinating seeds. A spectrophotometric assay showed no evidence for the presence of PAT in MS3 kernels.</p>
