<p style="text-align: justify;">Transgenic carnation line 66 was produced by <i>Agrobacterium</i>-mediated transformation of carnation plants (<i>Dianthus caryophyllus</i> L., cultivar Ashley) in which the transfer-DNA (T-DNA) region of the bacterial Ti plasmid was modified to contain a truncated copy of the ACC synthase encoding gene and the ALS encoding gene (<i>surB</i>) from a chlorsulfuron tolerant isolate of tobacco. The truncated ACC synthase gene was arranged in the &ldquo;sense&rdquo; orientation and contained promoter sequences from the 35S transcript of cauliflower mosaic virus (CaMV) and transcription termination and a 3&rsquo; polyadenylation signal sequence from the 3&rsquo; untranslated region of the <i>A. tumefaciens</i> nopaline synthase gene (3&rsquo; <i>nos</i>).</p>

<p style="text-align: justify;">The <i>surB</i> gene was used as a selectable marker to identify transformed plants during tissue culture regeneration. Expression of the ALS enzyme was regulated using the CaMV 35S promoter and the <i>surB</i> terminator derived from tobacco (<i>Nicotiana tabacum</i>).</p>
