<p style="text-align: justify;">Tobacco line Vector 21-41 was produced by <i>Agrobacterium</i>-mediated transformation of leaf discs from Burley 21 LA using the binary vector pYTY32. Transformed plantlets were regenerated on MS medium containing kanamycin as a positive selection agent.</p>

<p style="text-align: justify;">The plasmid vector pYTY32 was transformed into disarmed <i>Agrobacterium tumefaciens</i> strain LBA 4404 via electroporation. The plasmid pYTY32 was a modification of the pBin19 plasmid which and carried the NtQTP1 promoter and the antisense configuration of NtQTP1 cDNA As well, the plasmid T-DNA region contained the <i>nptII</i> gene coding for kanamycin resistance as a marker. Transcription of the <i>nptII</i> gene was regulated using the promoter and termination sequences from the <i>A. tumefaciens</i> nopaline synthase encoding gene (<i>nos</i>).</p>
