<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">As these were products of chemically induced mutagenesis, there was no introduction or incorporation of heterologous DNA into the rice genome. The tolerance to imidazolinone-containing herbicides is due to a single point mutation within the ALS encoding gene such that the amino acid sequence of the mutated enzyme differs by one amino acid from that of the wild-type enzyme.</p>

<p style="text-align: justify;"><b>Genetic Stability of the Introduced Trait</b></p>

<p style="text-align: justify;">Herbicide treatments performed over several generations demonstrated that the herbicide tolerance trait was stably inherited and expressed in events IMINTA 1 and IMINTA 4.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">There were no new novel proteins expressed. The level of expression of ALS within plant tissue is generally low (~0.001% of total protein in rice seed), and this level of expression was not altered because of the genetic modification. High pressure liquid chromotagraphy (HPLC) of protein extracts from unmodified and modified rice varieties did not indicate the expression of new proteins, or altered expression of existing proteins, as a result of the mutagenic event.</p>
