<p style="text-align: justify;"><b>The Inserted DNA</b></p>

<p style="text-align: justify;">Southern blot analyses of genomic DNA from FLAVR SAVR&trade; tomato line CR3-613 demonstrated insertion of the complete T-DNA region containing sequences encoding the antisense PG gene construct and NPTII. There was no incorporation of plasmid DNA sequences outside of the T-DNA region.</p>

<p style="text-align: justify;"><b>Genetic Stability of the Introduced Trait</b></p>

<p style="text-align: justify;">FLAVR SAVR&trade; tomatoes were tested for five generations, establishing that the T-DNA inserted into the plants was stable and remained unchanged from one generation of plants to the next.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The only novel protein expressed in FLAVR SAVR&trade; tomatoes was the enzyme NPTII. The antisense PG gene did not encode any polypeptide products and was introduced to suppress endogenous PG activity. The measured level of endogenous PG activity in transgenic FLAVR SAVR&trade; tomato was found to be less than 1% of PG activity found in the unmodified parental line.</p>
