<p style="text-align: justify;"><b>Dietary Exposure</b></p>

<p style="text-align: justify;">The human consumption of canola products is limited to the refined oil. Refined edible canola oil does not contain any detectable protein and consists of purified triglycerides (96-97%). As the introduced gene products are not detectable in the refined oil produced from transgenic canola, there will be no human exposure to these proteins based on normal consumption patterns.</p>

<p style="text-align: justify;"><b>Nutritional Data</b></p>

<p style="text-align: justify;">The analysis of nutrients from transgenic canola lines MS1 and RF1 and non-transgenic canola did not reveal any significant differences in the levels crude protein, crude fat, crude fibre, ash and gross energy in either whole seed or processed meal. The fatty acid composition of oils extracted from both transgenic and non-transgenic canola was statistically the same and within the normal range for canola oil. The transgenic lines meet the standards for canola oil in Canada of containing less than 2% erucic acid and less than 30 micromoles/g glucosinolates in the oil-free meal. It was determined that the consumption of refined oil from MS1, RF1 or hybrids derived therefrom would have no significant impact on the nutritional quality of the food supply in Canada.</p>

<p style="text-align: justify;"><b>Toxicity and Allergenicity</b></p>

<p style="text-align: justify;">Since only the processed oil from transgenic MS1, RF1, or hybrids derived therefrom (MS1xRF1), will be available for human consumption and the processing removes proteinaceous material, there are no additional toxicity or allergenicity concerns with this product. This was further demonstrated by examining the amino aid sequence homology and the characteristics of the novel proteins RNAse (<i>barnase</i>), RNAse inhibitor (<i>barstar</i>),PAT (<i>bar</i>) and neomycin phosphotransferase (NPTII).</p>

<p style="text-align: justify;">Comparative analyses with amino acid sequences of known protein toxins and allergens in public domain databases did not reveal any homologies. The only sequence homologies were with other related proteins. For example, <i>barnase</i> encoding RNAse was similar to ribonucleases from other bacilli, PAT was similar to other phosphinothricin acetyltransferases originating from different organisms, and NPTII was found to be similar to other peptides that have a similar function (aminoglycoside 3&#39;-phosphotransferases, kanamycin kinases, streptomycin 3&#39;-kinases. No resemblance with potential toxins or allergens was observed.</p>
