<p><b>Nutritional Data</b></p>

<p>Samples of grain and forage from transgenic and non-transgenic control lines were subjected to compositional analyses measuring the levels of protein, fat, ash, calcium and phosphorous. Forage samples were also analyzed for crude fibre content. For forage, the levels of analyzed components were found to be comparable between the male-sterile lines, between these lines and non-transgenic control lines, and to previously published literature values. With the exception of calcium levels, which were comparable between the male-sterile and non-transgenic control lines but lower than literature values, similar results were reported for the compositional analyses of grain samples. The lower calcium levels were attributed to an alteration in the analytical method.</p>

<p>Analyses of fatty acids (C16:0, palmitic; C18:0, stearic; C18:1, oleic; C18:2, linoleic; and C18:3, linolenic) and amino acids in samples from each male-sterile line and non-transgenic control lines did not reveal any significant differences in the respective value of each analyte. Except for the levels of a few amino acids, which were lower than those reported in the literature for commercial maize hybrids, the values measured for each analyte were within the range reported in the scientific literature. The differences noted in the levels of a few amino acids were likely due to the genetic background of the inbred parental lines relative to those lines analyzed in previously published studies.</p>

<p><b>Toxicity and Allergenicity</b></p>

<p>Previous studies have demonstrated that the PAT protein has a very low potential to be toxic or allergenic. The enzyme possesses none of the physiochemical characteristics commonly associated with known toxins or allergens, such as resistance to thermal or proteolytic degradation. The amino acid sequence of the PAT protein does not share homology with the sequences of known protein toxins and allergens, and in acute toxicity testing with laboratory mammals, adverse effects have never been observed.</p>
