<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">The novel herbicide tolerant trait introduced into the maize hybrid DK404SR was achieved through a somaclonal mutation within the maize genome. There was no new genetic material introduced into the maize hybrid DK404SR as a result of the modification.</p>

<p style="text-align: justify;"><b>Genetic Stability of the Introduced Trait</b></p>

<p style="text-align: justify;">Expression of the herbicide tolerance trait was consistently displayed by DK404SR plants that were at least six generations away from the original S2 line.</p>

<p style="text-align: justify;">The sethoxydim tolerance trait was inherited as a single partially dominant allele, as determined from the original mutant S2 line in which regenerated S2 plants were heterozygous for the mutant allele. The heterozygous progeny expressed herbicide tolerance at lower levels compared to homozygous plants.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The activity of the ACCase enzyme in unmodified maize and homozygous seedlings derived from the S2 line was shown to be similar in the absence of sethoxydim herbicide. Application of sethoxydim herbicide inhibited the activity of ACCase by 50% in modified maize plants when applied at a concentration 100 fold higher than the sethoxydim concentration required to achieve a similar level of inhibition of ACCase activity in unmodified maize plants.</p>
