<p style="text-align: justify;">Potato event EH92-527-1 was developed by <i>Agrobacterium tumefaciens</i>-mediated transformation of the cultivar Prevalent. T-DNA, from plasmid pHoxwG, derived from pBIN19, was delivered to leaf discs using a binary vector system. The introduced DNA included the <i>nptII</i> gene derived from the Tn5 transposon of <i>Escherichia coli</i> under the control of the nopaline synthase (<i>nos</i>) promoter and terminator, and a potato genomic <i>gbss</i> fragment oriented in the antisense direction, under the control of its own promoter and the <i>nos</i> terminator. The endogenous potato <i>gbss</i> gene encodes for granule-bound starch synthase (GBSS), an enzyme which is responsible for amylose biosynthesis. RNA transcription from the antisense <i>gbss</i> construct leads to a reduction in expression of the endogenouts potato <i>gbss</i> gene. <i>nptII</i> encodes neomycin phosphotransferase II, an enzyme that inactivates aminoglycoside antibiotics such as kanamycin, neomycin, and geneticin and was used as a selectable marker to identify transformed plants.</p>
