<p style="text-align: justify;">LLCotton25 was produced by <i>Agrobacterium</i>-mediated transformation of plant cells from the cotton variety &ldquo;Coker 312&rdquo;. The plasmid vector pGSV71 used for transformation contained the following: the gene coding for glufosinate ammonium tolerance (<i>bar</i> gene), the promoter region (P35S) from the cauliflower mosaic virus, and the 3&rsquo; untranslated end of the nopaline synthase gene (3&rsquo; <i>nos</i>). The transformation was achieved by culturing cotton tissue, excised between the hypocotyl and the radicle of three-day old cotton seedlings, with a culture of <i>A. tumefaciens</i> harbouring the Ti plasmid pGV3000 and the plasmid vector pGSV71. Explants from this culture were then regenerated to whole plants using tissue culture techniques. Transformed plants expressing the <i>bar </i>gene were selected with glufosinate ammonium.</p>
