<p style="text-align: justify;">These transgenic Russet Burbank potato lines were created through two separate <i>Agrobacterium</i>-mediated transformation events in which the transfer DNA (T-DNA) contained the genes encoding the Cry3A protein from <i>B. thuringiensis</i> subsp. tenebrionis and the ORF-1 and ORF-2 regions from PLRV. These two ORFs encode the putative viral helicase and replicase domains that are required for viral RNA synthesis. Regulation of the <i>cry3A</i> gene was via the ribulose-1,5-bisphosphate carboxylase (Rubisco) small subunit promoter from <i>Arabidopsis thaliana</i>, while the figwort mosaic virus (FMV) 35S promoter was used for PLRV derived sequences. In addition, the T-DNA contained sequences encoding the enzymes neomycin phosphotransferase II (NPTII) for events RBMT21-129 and RBMT21-350, or 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) for RBMT22-082. The respective expression of either NPTII or EPSPS activity in RBMT21 and RBMT22 transformation events was used as a selectable trait for screening transformed plants for the presence of the <i>cry3A</i> and PLRV replicase genes.</p>
