<p style="text-align: justify;">Canola line GT73 was produced by <i>Agrobacterium</i>-mediated transformation of the <i>B. napus</i> cultivar &#39;Westar&#39;. The T-DNA region of the Ti plasmid was &quot;disarmed&quot; by removal of <i>virA</i> genes, normally associated with the pathogenicity and disease-causing properties of <i>A. tumefaciens</i>, and replacement with the CP4 EPSPS encoding and <i>goxv247</i> genes. During transformation, the T-DNA portion of the plasmid was transferred into the plant cells and stably integrated into the plant&#39;s genome.</p>

<p style="text-align: justify;">GT73 was produced using plasmid PV-BNGT04 and contained the CP4 EPSPS gene and the <i>goxv247</i> gene. Constitutive expression of the CP4 EPSPS gene was regulated using the figwort mosaic virus 35S promoter and the 3&#39; end of the pea rbcS E9 gene (E9 3&#39;). The CP4 EPSPS gene was fused to a chloroplast transit peptide (CTP 2) sequence derived from the <i>Arabidopsis thaliana</i> to allow targeting of the newly translated EPSPS enzyme into the chloroplast, the site of aromatic amino acid biosynthesis and endogenous EPSPS and GOX activity.</p>

<p style="text-align: justify;">Similarly expression of <i>goxv247</i> gene was under control of the 35S promoter from a modified figwort mosaic virus and the 3&#39; end of the pea rbcS E9 gene (E9 3&#39;). Expression of the <i>goxv247</i> gene was targeted to the chloroplast by the action of the N-terminal of the small subunit 1A (SSU1A) of the ribulose 1,5-bisphosphate carboxylase chloroplast transit peptide of <i>A. thaliana</i> (CTP1).</p>

<p style="text-align: justify;">There was no incorporation of translatable plasmid DNA sequences outside of the T-DNA region. No antibiotic resistance marker genes were introduced into GT73.</p>
