<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">Southern blot analyses of genomic DNA from these transgenic rice lines revealed the integration of a single copy of the <i>bar</i> gene cassette into the LLRICE62 genome, and one complete plus one partial copy of the <i>bar</i> gene cassette into the LLRICE06 genome. No other plasmid encoded gene sequences were detected in the genomes of either of these transgenic lines.</p>

<p style="text-align: justify;"><b>Genetic Stability of the Introduced Trait</b></p>

<p style="text-align: justify;">The herbicide tolerant trait from lines LLRICE06 and LLRICE62 was introduced into commercial rice varieties via traditional backcrossing, and segregated as a single genetic locus in progeny T1 and T2 generations. Southern blot analyses of genomic DNA from T2, T3, and T4 generations of LLRICE06, and from T2 and T3 generations of LLRICE62, yielded identical hybridization patterns, confirming the genetic stability of the original transformation events.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The only newly expressed protein in the transformation events LLRICE06 and LLRICE62 was the PAT protein. The PAT protein expressed in these plants was characterized and found to be equivalent to the previously characterized reference standards.</p>

<p style="text-align: justify;">The levels of PAT protein expressed in rice grain and various processed fractions derived from transgenic plants was quantitated by enzyme linked immunosorbent assay (ELISA) and determined to be very low. The assay detected both inactive and intact enzyme, such that positive detection did not necessarily indicate functional protein.</p>
