<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">Southern blot analysis of genomic DNA from GT200 indicated the presence of a single site of insertion that contained one complete copy of the CP4 EPSPS gene and a complete copy of the <i>gox</i> gene and their respective regulatory sequences. The GOX protein introduced into canola line GT200 was shown to have a 99% similarity to the modified GOXv247 in transgenic line GT73. Both proteins are 46.1 KDa (there is a 17 Da difference) and differ by three amino acid substitutions.</p>

<p style="text-align: justify;"><b>Genetic Stability of the Introduced Trait</b></p>

<p style="text-align: justify;">Segregation analysis and Southern blots were consistent with a single site of insertion of the CP4 EPSPS and <i>gox</i> genes into the GT200 genome. GT200 was several generations removed from the original transformant and a comparative analysis of third-generation material, using Southern blots and PCR analysis, determined that the introduced DNA was stably inserted and stably inherited of several generations.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The expression of both CP4 EPSPS and GOX was quantified in the leaves and seeds of canola line GT200. Enzyme linked immunosorbent assay (ELISA) was used to determined average levels of CP4 EPSPS and GOX as follows: in seeds 31 &micro;g/g CP4 EPSPS (f.w.) and 105 &micro;g/g GOX (f.w); and in leaves 31 &micro;g/g CP4 EPSPS and 69 &micro;g/g GOX (f.w.)</p>
