<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">Southern blot analyses of genomic DNA from MON 863 demonstrated that there was a single site of integration containing one functional copy each of the modified <i>cry3Bb1</i> and <i>nptII</i> genes and their associated regulated regulatory sequences. No deletions, truncations, or rearrangements were reported, nor were any additional plasmid-derived sequences detected.</p>

<p style="text-align: justify;"><b>Genetic Stability of the Introduced Trait</b></p>

<p style="text-align: justify;">Segregation and stability of the introduced trait were examined by Southern blot analyses performed on genomic DNA extracted from plants spanning three generations, and by enzyme linked immunosorbent assay (ELISA) to detect expression of the Cry3Bb1 protein over five generations of progeny plants. These results demonstrated that the introduced trait continued to segregate as a single locus, and that protein expression remained stable over multiple generations.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The levels of expression of Cry3Bb1 protein in various plant tissues, including young leaf, grain, mature root, forage, silk, and pollen, were measured by ELISA and were found to vary between 10 to 81 micrograms/g fresh weight of plant tissue, depending on the tissue type and the time of harvest. Expression of the NPTII protein was also confirmed in young leaf, forage, and grain, obtained from MON 863, with levels ranging from undetectable to 1.4 micrograms/g when measured by ELISA (limit of detection: &lt;=0.076 micrograms/g).</p>
