<p style="text-align: justify;">Pioneer used microprojectile bombardment to co-transform secondary plant cell embryos with two purified linear DNA fragments: a 2924 base pair fragment (PHP19340A fragment) containing the <i>gm-fad2-1</i> cassette, and the 4512 base pair fragment (PHP17752A fragment) containing the <i>gm-hra</i> cassette. The <i>gm-fad2-1</i> cassette includes the promoter region from the soybean Kunitz trypsin inhibitor gene (<i>KTi3</i>), a fragment of the <i>FAD2-1</i> gene that corresponds to approximately 40% of the middle portion of the <i>FAD2-1</i> gene (597 bp), and the 3&#39; untranslated region of the <i>KTi3</i> gene (<i>KTi3</i> terminator).</p>

<p style="text-align: justify;">The <i>gm-hra</i> cassette contains the <i>gm-hra</i> gene (1971 bp), which is an optimized form of the endogenous <i>als</i> gene from soybean, with transcription regulated by the S-adenosyl-L-methionine synthetase (SAMS) constitutive promoter (1.3 kb) from soybean and with transcription terminated by the endogenous <i>als</i> gene terminator (0.6 kb) from soybean.</p>
