<p style="text-align: justify;">The cotton line 3006-210-23 was produced by <i>Agrobacterium</i>-mediated transformation of plant cells from the cotton variety &lsquo;Germain&rsquo;s Acala GC510&rsquo; using the the binary plasmid vector pMYC3006. The plasmid contained the <i>cry</i>1Ac gene, coding for a full length chimeric Cry1Ac protein (delta-endotoxin) which confers lepidopteran insect resistance; a <i>Zea mays</i> promoter system (UbiZm1); and a bi-directional terminator (ORF25polyA) from <i>A. tumefaciens</i> strain LBA 4404 pTi15955. The pMYC3006 plasmid also contained a synthetic version of the <i>pat</i> gene, coding for glufosinate ammonium tolerance, and used as a selectable marker. The expression of the <i>pat</i> gene was controlled by a mannopine synthase promoter from pTi15955, and four copies of the octopine syntase (4OCS) enhancer from pTiAch5. Polyadenylation sequences were derived from the bi-directional ORF25 terminator from pTi15955. The plasmid backbone, derived from plasmid Rk2, contained an erythromycin resistance gene to allow the selection of bacteria containing pMYC3006.</p>

<p style="text-align: justify;">Successful transformants were detected as those tolerant to glufosinate ammonium. Resistance to lepidopteran insects was tested by conducting bioassays using leaf discs from the successful transformants. These were fed to cotton bollworm, a target lepidopteran pest. The successful event was designated 3006-210-23 and was subsequently introgressed into the elite genotype &lsquo;PSC355,&rsquo; a cultivar with a broad adaptation to the southern United States.</p>
