<p style="text-align: justify;"><b>The Introduced DNA</b></p>

<p style="text-align: justify;">Southern blot analysis of genomic DNA from CZW-3 indicated the presence of a single copy of the T-DNA containing intact sequences corresponding to the CP encoding genes form CMV, ZYMV, and WMV2, integrated at a single site. Additional analyses verified the presence of a single copy of the NPTII encoding <i>neo</i> antibiotic resistance marker gene, and no incorporation of plasmid backbone sequences outside of the T-DNA region.</p>

<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The level of expression of the CMV coat protein in transgenic squash was quantitated by enzyme linked immunosorbent assay (ELISA) and estimated to be 5.9-10.8 ng/g fruit tissue, which was of the same order of magnitude as the concentrations of ZYMV and WMV-2 coat proteins measured in the related squash line ZW-20. These concentrations were significantly lower those measured in naturally virus infected samples of zucchini squash, yellow crookneck squash, cantaloupe and honeydew melons obtained from supermarket shelves. The level of expression of NPTII (44 &micro;g/g fruit tissue) was judged to be insignificant with respect to any human health risk due to exposure.</p>
