<p style="text-align: justify;">Event T120-7 was produced by <i>Agrobacterium</i>-mediated transformation of calli from the parent line R01 with plasmid vector pOCA18/Ac. The transfer-DNA (T-DNA) portion of the tumour inducing (Ti) bacterial plasmid was engineered to contain a modified form the PAT encoding gene as well as the <i>nptII</i> gene. In order to enhance plant expression of the protein, the nucleotide sequence of the <i>pat</i> gene was modified using site-directed mutagenesis to contain plant-preferred codons. These modifications did not result in changes to the predicted amino acid sequence of the PAT enzyme. Expression of NPTII was used as a selectable marker to screen for transformed plants during tissue culture regeneration and multiplication.</p>

<p style="text-align: justify;">Associated with the <i>pat</i> gene were promoter sequences, as well as transcription termination and polyadenylation signal sequences, derived from the 35S transcript of cauliflower mosaic virus (CaMV). Expression of the NPTII encoding gene was regulated using promoter sequences from the <i>A. tumefaciens</i> nopaline synthase encoding gene (<i>nos</i>).</p>
