<p style="text-align: justify;"><b>Expressed Material</b></p>

<p style="text-align: justify;">The expression levels of Cry1Ac, nitrilase, and NPTII were quantitated by Western immunoblot analysis of samples of cottonseed and meal. The estimated concentrations of the three proteins in seed were 2.5 ppm, 0.25 ppm and 2.5 ppm, respectively, on a fresh weight basis. For samples of meal, the values were 2.25 ppm, 1.4 ppm and 13.5 ppm for Cry1Ac, nitrilase, NPTII, respectively. None of these proteins were detected in samples of oil from cotton lines 31807 and 31808 at a detection limit of 0.01 ppm.</p>
