The possibility of gene flow between Solanum species was tested through crossing 54 accessions from 13 Solanum species (seven wild and six cultivated), both in open pollinated fields in several areas of the Peruvian Andes and in greenhouse crosses.  In this paper, the results of the greenhouse crosses are reported and only the Puno open pollinated field results, both with AFLP fingerprinting, as proof of hybridity and identification of the pollen parent. The cultivated species were selected on the basis of commonly grown native or hybrid varieties and wild species found near or around cultivated potato fields in five different agroe-ecogeographies of Peru, above 3200 m asl. Results from greenhouse and field crosses can be divided in two main groups:1. Same ploidy and EBN number2. Different ploidy and/or EBN