In this paper, we examine awareness of biotechnology and the attitudes towards products and services derived from Genetically Modified Organisms (GMOs) in Southwest Nigeria. For this purpose, we surveyed 180 respondents across selected segments of the regional population. The paper demonstrates that while respondents have some awareness of biotechnology techniques, there is little awareness about specific GM crops that include Bt maize, Bt cotton and golden rice. Most of the respondents tend to be in favour of the introduction of GM crops and would eat GM foods if they are more nutritious than non-GM foods. The attitudes of the respondents towards GMOs suggest that though more people are in favour of the introduction of GM crops, they do not consider the current state of Nigeria's institutional preparedness satisfactory for the approval and release of GMOs.