Corn plants (Zea mays L.) have been genetically engineered for protection from damage by the European Corn Borer (ECB, Ostrinia nubilialis), a major corn pest. Protection was accomplished by insertion of a gene from Bacillus thuringiensis subsp. Kurstaki (B.t.k.) which encodes for the production of a protein(B.t.k. HD-1) which is insecticidal to Lepidoptera larvae. In support of regulatory approval of this ECB protected corn, data and information was obtained to demonstrate that B.t.k. HD-1 protein expressed by the inserted gene causes no adverse effects when consumed by domestic or wild animals or beneficial insects. Because the expressica levels of the B.t.k. HD-1 protein are extremely low in ECB protected corn, it was not feasible to isolate this protein from plants in sufficient quantity to conduct the various safety studies performed for the registration of this product.