Studies were conducted to assess the impact of various Integrated Pest Management (IPM) practices on natural enemy complex in Bt cotton (MECH-184 Bt) at Regional Agricultural Research Station (RARS), Raichur and in farmers fields. The results revealed a significantly higher predator population of Chrysopids (eggs and larvae), coccinellids (grubs and adults), anthocorids (adults) and spiders on MECH-184 Bt cotton compared to NCS-145 (Bunny, local popular hybrid). The increased population of natural enemies in Bt cotton was attributed to lesser number of insecticidal sprays and prevalence of sucking pests which served as food for predators. The utilization of natural enemies in transgenic cotton cultivation would be valuable.