Genetically modified organisms (GMOs) has been the center of debates among the food producer and consumer worldwide. The issue arised in Thailand is not different especially the concern of the safety of food that derived from GMOs and their product. Livestock industry has been subjected as the user of GMOs in the production system. To lessen some of these controversial issue, this study was aimed at detection of the GMOs in broiler that utilized GMOs soybeanmeals (SBM) as a feed ingredient. The broiler were divided into 3 groups which allocated for the 3 diets. All diets formulated to meet the commercial standard were diet1 as non-GMO SBM, diet2 as GMO SBM and diet3 as the commercial diet that was GMOs. Broilers were fed in a commercial management from birth up to 7 week olds. Samples of broilers (meat, skin, duodenum and liver) were taken at 1, 3, 5 and 7 weeks. Real-time PCR (ABI Prism 7700) were used to analyze contamination of GMOs in all samples. PCR results of the broiler samples during 1-7 week were all negative. The results implied that the GMOs content in SBM has been metabolized in the gut of the broiler and show no residue in the meat. Therefore, other aspects of the safety issues are needed to verify scientifically.