Compositional analyses and safety assessments of glyphosate-tolerant soyabeans (GTS) have been previously described. These analyses were extensive and included addressing the potential effects of the genetic modification on seed composition. Detailed compositional analyses established that GTS which had not been treated with glyphosate were comparable to the parental soyabean line and to other conventional soyabeans. In this study, two GTS lines, 40-3-2 and 61-67-1, were treated with commercial levels of glyphosate, the active ingredient in Roundup herbicide. The composition of the seed from soyabeans sprayed with glyphosate was compared to that from the non-sprayed parental control cultivar, A5403. The nutrients measured in the seed included protein, oil, ash, fibre, carbohydrates and amino acids. The concentration of isoflavones (also referred to as phytoestrogens) was also measured, as these compounds are derived from the same biochemical pathway that was engineered for glyphosate tolerance. The analytical results from these studies demonstrated that the GTS treated with glyphosate were comparable to the parental soyabean cultivar A5403 and other conventional soyabean varieties.