Defatted flakes or flour obtained from 2 glyphosate tolerant soyabean lines, 40-3-2 and 61-67-1, and the parental control variety were ground to a fine powder and then subjected to SDS-PAGE and immunoblotting. The electrophoretic banding patterns obtained for the glyphosate tolerant lines were identical to that of the parental control. Immunoblotting using a serum pool containing soyabean allergen-specific IgE antibody, generated from 5 patients with atopic dermatitis and soyabean hypersensitivity, revealed no differences in IgE-binding protein patterns between the different soyabean genotypes. Immunoblots with IgE antibody pools obtained from groundnut sensitive or non-atopic patients showed that a minimal amount of non-specific binding occurred at approximately 45 and 50 kDa for all the soyabean preparations. The results demonstrated that introduction of a gene for glyphosate tolerance caused no discernible changes, either quantitative or qualitative, in the composition of endogenous allergenic proteins in soyabean.