The planting area of transgenic herbicide-resistant crops accounted for more than 80% of the total planting area of transgenic crops in 2008. Herbicide resistance becomes the major one among all the transgenic traits that has been engineered into crops. The application of transgenic herbicide-resistant crops results in the drop in weed control cost, the decrease of herbicide residue, and the increase crop safety. However, large-scale commercialization of transgenic herbicide-resistant crops may lead to uniform of herbicidal market, thus exert great influences on herbicidal industry development. In addition, environmental safety issues, such as herbicide resistant weed evolution and transgene escape, may be involved. Therefore, the development of herbicide-resistant crops faces a variety of challenges. However, development of transgenic herbicide-resistant crops, in spite of not commercialized yet in China, is meeting a good development opportunity due to huge potential market and policy support. In addition, this paper addresses how to use herbicide-resistant gene reasonably, and the research and development strategy for transgenic herbicide-resistant crop in order to provide beneficial information for the research and development of transgenic herbicide-resistant crops in China.