Varietal purity in wheat (Triticum aestivum L.) seed production is necessary for agronomic uniformity and to enable potential market segregation. We conducted a survey of certified and farm-saved seed samples using a nontransgenic imidazolinone-resistant (IR) wheat cultivar in 2004 and 2005 in eastern Colorado. The objective was to compare varietal purity based on type of seed producer and IR wheat history. Ninety-two samples of non-IR varieties were taken from certified and farm-saved seed growers, who either produced or had never produced IR wheat. Adventitious IR seeds were detected using a seed-soaking technique in samples from each producer type and each IR production history. Levels of IR seed ranged from 0 to 11.28%. One certified sample and three farm-saved samples exceeded the 0.1% threshold for off-types in certified wheat seed. Using a two-factor analysis, farm-saved production class and positive IR history increased the estimated proportion of adventitious seed. Based on grower interviews, higher levels of adventitious seed presence were associated with volunteer plants from previous crops of the resistant cultivar and mechanical mixture during harvesting. Production practices for certified seed address these factors and may need to be strengthened if more stringent purity criteria are adopted. This information is important for risk assessment and policy development for potential commercial release of transgenic wheat varieties.