To evaluate potential environment safety for release of transgenic rice  Minghui 86B, the potential ecological risk of weediness of transgenic glufosinate-resistant rice Minghui 86B was assessed. The surviving and reproduction ability&amp;#65292;seed shattering, seed viability of transgenic glufosinate-resistant rice Minghui 86B, Minghui 86 and hybrid rice Shanyou 63 were comparatively evaluated in field, as well as their tolerance to flucetosulfuron in greenhouse. The surviving   and reproducing abilities of Minghui 86B and Minghui 86 were lower than that of Shanyou 63 both in the appropriate season and in the unfavorable season, especially in the latter. The surviving ability of Minghui 86B was lower than that of Minghui 86. The seed shattering of the three rice materials were weak, and no volunteer seedlings were found. After shallow and deep buried, the seed viability of transgenic rice was weaker than those of Shanyou 63 and Minghui 86. The tolerance of the three rice materials to herbicides flucetosulfuron was similar. It is concluded that potential weediness of the transgenic glufosinate-resistant rice Minghui 86B, was low in Nanjing, China.